天津医药

• 实验研究 •    

高频电刺激脚桥核对苍白球内侧部氨基酸类递质及神经元放电的影响

刘敏杰   

  1. 辽宁锦州， 辽宁医学院生理学教研室
  • 收稿日期:2012-12-11 修回日期:2013-03-25 出版日期:2013-07-15 发布日期:2013-07-15
  • 通讯作者: 刘敏杰

Effect of High-Frequency Stimulation in the Pedunculopontine Nucleus on Neuronal Activity and Neurotransmitters in the Globus Pallidus Internus of Rats

Liu Min Jie   

  1. Department of Physiology, Liaoning Medical College, Jinzhou 121000, China
  • Received:2012-12-11 Revised:2013-03-25 Published:2013-07-15 Online:2013-07-15
  • Contact: Liu Min Jie

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摘要：

【摘要】 目的  通过观察高频电刺激帕金森(PD)模型大鼠脚桥核(PPN)对苍白球内侧部(GPi)氨基酸类递质及神经元放电的影响,探讨电刺激PPN治疗PD的机制。方法  将70只SD雄性大鼠随机分为对照组30只,PD模型组40只。大脑右侧黑质致密部两点注射6-羟基多巴胺建立PD模型,应用细胞外单位记录观察高频电刺激PPN对GPi神经元放电的影响。脑内微透析收集损毁侧GPi脑脊液,结合高效液相色谱检测氨基酸类递质的变化。结果  高频电刺激PPN,对照组及PD模型组大鼠GPi神经元的反应均以兴奋性为主,且平均放电频率均较刺激前明显增高(P﹤0.01);高频电刺激PPN,GPi内谷氨酸水平较刺激前明显减少(P﹤0.01),γ-氨基丁酸水平较刺激前无明显变化(P﹥0.05)。结论  高频电刺激PPN可增强GPi神经元的电活动,降低GPi内谷氨酸的水平,其兴奋GPi神经元的效应可能是通过PPN-GPi直接通路或其他间接通路实现的。 

关键词: 帕金森病, 疾病模型, 动物, 羟多巴胺, 电刺激, 苍白球, 谷氨酸, γ氨基丁酸

Abstract: Objective   To study the effect of high frequency stimulation (HFS) of pedunculopontine nucleus (PPN) on the neuronal activities of globus pallidus internus (Gpi) in Parkinson’s disease (PD) model rats and measure the level of amino acids neurotransmitter. Methods   Seventy male Sprague-Dawley rats were divided into two groups, control group(n=30) and PD model group(n=40). PD model rats were established by the injection of 6-OHDA into SNc with stereotactic technique on the right side of the brain. Electrophysiological recordings were made in anaesthetized rats to investigate the effects of HFS-PPN on the firing rate of the GPi neurons. Brain microdialysis combined with high-performance liquid chromatography was applied to detect glutamate (Glu) andγ-aminobutyric acid (GABA) levels in the GPi. Results   HFS-PPN caused an excitatory reaction of the majority of neurons recorded in the GPi in the PD rats and control group rats, the mean firing rate of GPi excited neurons was significantly increased; The levels of Glu were reduced under HFS-PPN and the levels of GABA were not affected. Conclusion    HFS-PPN heightened the electrical activity of GPi neurons and reduced the level of Glu. This excitatory effects were probably realized by PPN-GPi direct path or other indirect path.

Key words: Parkinson disease, Disease Models, Animal, oxidopamine, Electrical stimulation, globus pallidus, glutamic acid, γ氨基丁酸