关键词: 癌, 非小细胞肺； 铁螯合剂； 顺铂； 细胞增殖； 细胞凋亡； 地拉罗斯

Abstract: Abstract: Objective To investigate the combination effect using iron chelators deferasirox and cisplatin on A549 cell proliferation and apoptosis and to provide evidences to explore an effective way to treat lung cancer. Methods Lung adeno⁃ carcinoma cells were cultured by conventional way, with administration of different concentrations of deferasirox and cisplat⁃ in. Cell growth inhibition was observed under an inverted microscope. Proliferation inhibition was evaluated by MTT assay. Morphological changes of cell apoptosis was detected using DAPI,AO/EB straning and flow cytometry. Results After a cer⁃ tain time of incubation with different concentrations of the combined drugs，the cell number reduce significantly, which was counted under invert microscope. Cells were dispersed with each other and adherent cells appear shrunken and poor in re⁃ fractivity. MTT assay showed that inhibition of cell proliferation was in a concentration-time-dependent manner. Chromatin condensation, nuclear condensation and other typical apoptotic morphology were detected after DAPI and AO/EB straning. Flow cytometry showed that apoptosis increased with rising drug concentration. So combination therapy was significantly pro-apoptotic. Conclusion Deferasirox has the ability to inhibit proliferation of A549 cells and can promote tumor cell apoptosis and enhances cancer cell tolerance when combined with cisplatin. It can also reduce the amount and toxicity of cis⁃ platin. It provides a basis for finding an effective way to treat lung cancer.

Key words: carcinoma, non-small-cell lung, iron chelating agents, cisplatin, cell proliferation, apoptosis； deferasirox