天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (4): 344-347.doi: 10.11958/j.issn.0253-9896.2015.04.003

• 细胞与分子生物学 • 上一篇    下一篇

碱性成纤维细胞生长因子对人牙龈成纤维细胞成骨分化与增殖的影响

甄珍，蒋少云，陶玉飞，严志敏，邓嘉胤△   

  1. 天津医科大学口腔医院（邮编300070）
  • 收稿日期:2014-07-31 修回日期:2014-12-24 出版日期:2015-04-15 发布日期:2015-04-13
  • 通讯作者: 邓嘉胤 E-mail:zguxingleiz@163.com
  • 作者简介:甄珍（1989），女，硕士研究生在读,主要从事牙周组织工程的研究
  • 基金资助:
    天津市应用基础及前沿技术研究计划（自然科学基金重点项目）（12JCZDJC22700）

Effect of basic fibroblast growth factor on osteogenic differentiation and cell proliferation of human gingival fibroblasts in vitro

ZHEN Zhen, JIANG Shaoyun, TAO Yufei, YAN Zhimin, DENG Jiayin△   

  1. Department of Periodontology, Stomatology Hospital of Tianjin Medical University, Tianjin 300070, China
  • Received:2014-07-31 Revised:2014-12-24 Published:2015-04-15 Online:2015-04-13
  • Contact: DENG Jiayin E-mail:zguxingleiz@163.com

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摘要： 摘要：目的观察碱性成纤维细胞生长因子（bFGF）对体外培养的人牙龈成纤维细胞（HGFs）成骨分化能力与细胞增殖的影响，探索bFGF 在HGFs 体外诱导成骨分化过程中的作用。方法采用组织块贴壁法体外培养HGFs，取第3 代细胞进行如下分组培养。1 组为普通培养基组，2 组为普通培养基+10 μg/L bFGF 组，3 组为成骨诱导组，4 组为成骨诱导+10 μg/L bFGF 组。应用四甲基偶氮唑蓝比色法检测HGFs 增殖状况；用碱性磷酸酶染色法及茜素红染色法检测HGFs 的成骨分化能力。结果在普通培养基和成骨诱导培养基中，10 μg/L 的bFGF 均能促进HGFs 的增殖（P＜0.01）；在成骨诱导培养基中HGFs 具有骨向分化能力，形成钙结节；而10 μg/L 的bFGF 对HGFs 的碱性磷酸酶活性与矿化结节形成能力均无明显影响。结论10 μg/L 的bFGF 能促进HGFs 的增殖能力，而对其骨向分化无明显影响。

关键词: 成纤维细胞生长因子2, 成纤维细胞, 牙龈, 骨生成, 细胞分化, 细胞增殖

Abstract: Abstract: Objective To observe the effects of basic fibroblast growth factor (bFGF) on osteogenic differentiation abili⁃ ty and cell proliferation of human gingival fibroblasts (HGFs), and to explore the role of bFGF on the process of osteogenic differencitiaion in vitro. Methods HGFs were cultured in vitro until the 3rd passage when they were divided into four groups: normal medium as group 1, normal medium with 10 μg/L bFGF as group 2, osteogenic medium as group 3 and osteo⁃ genic medium with 10 μg/L bFGF as group 4. MTT assay was used to evaluate the proliferation of HGFs. Alkaline phospha⁃ tase (ALP) staining and Alizarin red staining were applied to investigate osteogenic potential of HGFs under different culture conditions. Results bFGF at concentration of 10 μg/L could increase HGFs proliferation in both normal and osteogenic medium (P＜0.01). HGFs could be induced towards osteogenic differentiation and form mineralized nodule in osteogenic me⁃ dium. However, 10 μg/L bFGF had no effects on ALP activity and mineralized nodule formation of HGFs during osteogenic differentiation. Conclusion bFGF could promote the proliferation of HGFs but show no effects on osteogenic differentiation of HGFs at concentration of 10 μg/L.

Key words: fibroblast growth factor 2, fibroblasts, gingiva, osteogenesis, cell differentiation, cell proliferation