天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (6): 635-638.doi: 10.11958/j.issn.0253-9896.2015.06.015

• 临床研究 • 上一篇    下一篇

自噬基因 pULK、 PI3KC3 在非小细胞肺癌中的表达及相关性研究

吴楠 1， 闵鹤鸣 2， 屈惠莹 1， 包翠芬 2   

  1. 1锦州， 辽宁医学院人体解剖教研室 （邮编 121000）， 2分子细胞生物与新药开发省高校重点实验室
  • 收稿日期:2014-08-26 修回日期:2015-01-09 出版日期:2015-06-15 发布日期:2015-06-10
  • 通讯作者: 包翠芬 E-mail:cuifenbao1972@hotmail.com
  • 基金资助:
    辽宁省科技厅基金资助项目 （201302143）

Expression autophagy-related gene pULK and PI3KC3 and their correlation with human nonsmall-cell carcinoma

WU Nan1, MIN Heming2, QU Huiying1, BAO Cuifen2   

  1. 1 Department of Human Anatomy & Histology and Embryology, 2 Key lab of Molecular Cell Biology and New Drug
    Development, Liaoning Medical University
  • Received:2014-08-26 Revised:2015-01-09 Published:2015-06-15 Online:2015-06-10

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摘要： 目的 探讨自噬基因 pULK、 PI3KC3 在非小细胞肺癌（NSCLC）中的表达及相关性。方法 随机抽取NSCLC 患者 （肺癌组） 77 例 （鳞癌 31 例、 腺癌 31 例、 大细胞未分化癌 15 例） 及其癌旁正常组织 21 例作为对照。采用免疫组织化学染色及免疫印迹方法定性、 定量检测 pULK、 PI3KC3 在 NSCLC 和癌旁正常肺组织中的表达情况， 并采用 SPSS 13.0 统计软件进行病理因素及相关性分析。结果 免疫组化结果显示 pULK 和 PI3KC3 阳性表达定位于细胞质。pULK 和 PI3KC3 在肺癌组中的阳性表达(35.1%、 40.3%)显著低于癌旁正常肺组织(81.0%、 76.2%， P＜0.01)。NSCLC 中无淋巴结转移、 TNM 分期的Ⅰ～Ⅱ期和中高分化患者的 pULK 和 PI3KC3 蛋白表达阳性率均较高（P＜0.01）， 而不同年龄、 性别、 肿瘤直径大小的 NSCLC 的表达差异无统计学意义。相关性分析显示 pULK 与 PI3KC3 表达呈正相关关系。结论 pULK、 PI3KC3 在 NSCLC 中呈低表达。其表达与患者的临床分期、 分化程度及淋巴结转移有关， 而与患者年龄、 性别、 肿瘤组织学类型及大小无关。

关键词: 自噬, 磷酸转移酶类(醇族体), 癌, 非小细胞肺, 自噬相关蛋白 1, Ⅲ型磷脂酰肌醇 3 激酶

Abstract: Objective To examine expression levels of autophagy gene pULK and PI3KC3 and to explore their correlation with non-small cell lung cancer (NSCLC). Methods A total of 77 samples of surgical resection from NSCLC specimens (including 31 cases of squamous cell carcinoma, 31 cases of adenocarcinoma and 15 cases of large cell undifferentiated carcinoma) and 21 samples of same normal lung tissue were randomly selected. Expressions of pULK and PI3KC3 in lung tissues were assessed by immunohistochemistry and Western blot. All dates were analyzed using SPSS13.0 statistical package. Results Immunohistochemistry indicated that pULK and PI3KC3 localized into the cytoplasm. The expression levels of pULK and PI3KC3 are significantly lower in patient with NSCLC than those in peri-tumor tissue (35.1% vs 81.0%, 40.3% vs 76.2% respectively, P＜0.01) . Immunohistochemistry and Western bolt analysis confirmed that pULK and PI3KC3 expressions were significantly down-regulated (P＜0.01) in patients with low grade cellular differentiation,metastasis of lymph node, or stage Ⅲ and Ⅳ. And expression levels of pULK and PI3KC3 in NSCLC did not differ significantly with ages, gender, tumor size and pathological type. Correlation analysis showed that the expression of PI3KC3 was positively correlated with pULK. Conclusion pULK and PI3KC3 expressions were lower in NSCLC than those in normal lung tissue group. The expression levels of pULK and PI3KC3 in NSCLC were correlated with patient's clinical stage, differentiation grade, lymph node metastasis but were unrelated with age, gender, histological type and size.

Key words: autophagy, phosphotransferases (alcohol group acceptor), carcinoma, non-small-cell lung, autophagy related protein 1, phosphoinositide-3-kinase class 3