天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (7): 721-723.doi: 10.11958/j.issn.0253-9896.2015.07.005

• 细胞与分子生物学 • 上一篇    下一篇

盐酸戊乙奎醚对肺泡Ⅱ型上皮细胞氧化损伤的影响

杜学柯，潘灵辉，裴圣林，葛万运   

  1. 广西医科大学附属肿瘤医院麻醉科 （邮编 530021）
  • 收稿日期:2014-08-21 修回日期:2015-02-12 出版日期:2015-07-15 发布日期:2015-07-15
  • 通讯作者: 杜学柯 E-mail:duke2306@163.com
  • 作者简介:杜学柯 （1981）， 男， 主治医师， 主要从事呼吸急救方面研究
  • 基金资助:
    广西壮族自治区自然科学基金青年基金项目 （2013GXNSFBA019182）

Protective effects of penehyclidine hydrochloride on A549 cells against oxidative injury

DU Xueke, PAN Linghui, PEI Shenglin, GE Wanyun   

  1. Affiliated Hospital of Guangxi Medical University, Nanning 530021, China
  • Received:2014-08-21 Revised:2015-02-12 Published:2015-07-15 Online:2015-07-15
  • Contact: DU Xueke E-mail:duke2306@163.com

PDF (PC)

3541

摘要： 目的 探讨盐酸戊乙奎醚对肺泡Ⅱ型上皮细胞氧化损伤的保护作用。方法 用过氧化氢（H2O2）加入A549 细胞培养液中制成 A549 细胞氧化损伤模型， 实验分为空白对照组（C 组）、 H2O2处理组（H 组）和盐酸戊乙奎醚-过氧化氢处理组 （P 组）。用 MTT 方法测 A549 细胞存活率， 用 TUNEL 方法测定细胞凋亡指数， 并测定细胞内的丙二醛（MDA）、 超氧化物歧化酶（SOD）、 还原型谷胱甘肽（GSH）、 烟酰胺腺嘌呤二核苷磷酸（NADPH）含量。结果 与 C 组比较， H 组的细胞存活率下降， 凋亡指数增加， MDA 含量上升， SOD、 GSH、 NADPH 的含量下降 （P＜0.05）。与H 组比较， P 组的细胞存活率增加， 凋亡指数下降， MDA 含量下降， SOD、 GSH、 NADPH 的含量增加（P＜0.05）。结论 盐酸戊乙奎醚对A549 细胞过氧化损伤具有保护作用。
 

关键词: 盐酸戊乙奎醚, 肺泡Ⅱ型上皮细胞, A549 细胞, 氧化损伤

Abstract: Objective To explore the effects of penehyclidine hydrochloride (PHC) on lung epithelial type Ⅱ cells against oxidative stress damage. Methods A549 cells treated with H2O2 were used as oxidative stress damage cell model. A549 cells were divided into 3 groups: control group (C group), H2O2 treated group (H group) and PHC treated group (P group). The viability of A549 cells was measured by MTT assay. The apoptotic rate was measured by TUNEL assay. The levels of malonicdialed (MDA), reactive oxygen species (SOD), reduced glutathione hormone (GSH) and nicotinamide adenine dinucleotide phosphate (NADPH) of cells were detected by biochemistry colorimetry. Results Compared with group C, the viability of A549 and the contents of SOD, GSH and NADPH were significantly decreased in group H, while MDA content and apoptotic rate were increased (P＜0.05). Compared with group H, the viability of A549, the contents of SOD, GSH and NADPH were significantly increased in group P, while MDA content and apoptotic rate were reduced (P＜0.05). Conclusion Penehyclidine hydrochloride shows protective effects on A549 cells through reducing the oxidative damage induced by H2O2.

Key words: penehyclidine hydrochloride, lung epithelial type Ⅱcells, A549, oxidative stress damage