天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (12): 1364-1367.doi: 10.11958/j.issn.0253-9896.2015.12.006

• 细胞与分子生物学 • 上一篇    下一篇

肝细胞癌组织HBXIP 和GRIM-19 表达及意义的探讨

孙纪三，张建军，高伟，杨涛，郭庆军，马楠，王洪海，蒋文涛△   

  1. 天津市第一中心医院东方器官移植中心（邮编300192）
  • 收稿日期:2014-04-09 修回日期:2014-08-11 出版日期:2015-12-15 发布日期:2015-12-11
  • 通讯作者: △通讯作者E-mail：jiang-wentao@medmail.com.cn E-mail:wtgfcn@163.com
  • 作者简介:孙纪三（1980），男，主治医师，硕士，主要从事肝脏移植方面研究
  • 基金资助:
    天津市科技计划项目（10ZCGYSF00500）

Expressions of HBXIP and GRIM-19 in hepatocellular carcinoma and their clinic significance

SUN Jisan, ZHANG Jianjun, GAO Wei, YANG Tao, GUO Qingjun, MA Nan, WANG Honghai, JIANG Wentao△   

  1. Tianjin First Central Hospital of Oriental OrganTransplant Center，Tianjin 300192，China
  • Received:2014-04-09 Revised:2014-08-11 Published:2015-12-15 Online:2015-12-11
  • Contact: △Corresponding Author E-mail：jiang-wentao@medmail.com.cn E-mail:wtgfcn@163.com

PDF (PC)

3874

摘要： 目的探讨乙肝病毒X 蛋白结合蛋白HBXIP 和细胞凋亡调节因子GRIM-19 在肝细胞癌组织中的表达及意义。方法收集42 例肝细胞癌组织和28 例正常肝脏组织，采用免疫组织化学法检测两种组织中HBXIP 和 GRIM-19 的表达。结果在肝细胞癌组织和正常肝脏组织中，HBXIP 阳性表达率分别为80.95%（34/42）和42.86% （12/28）；而GRIM-19 阳性表达率分别为40.48%（17/42）和75.00%（21/28），差异有统计学意义（P＜0.01）。肝细胞癌组织中HBXIP 的阳性表达率在低、未分化和Ⅲ～Ⅳ期的表达水平高于高、中分化和Ⅰ～Ⅱ期组织；而GRIM-19 的阳性表达率与之相反，且无门脉癌栓组织中GRIM-19 的阳性表达率高于有门脉癌栓组织。HBXIP 与GRIM-19 表达呈负相关（rs=-0.400，P＜0.01）。结论HBXIP 和GRIM-19 的异常表达可能与肝细胞癌的发生及浸润转移有密切关系。

关键词: 癌, 肝细胞, 肿瘤转移, 免疫组织化学, 乙肝病毒X 蛋白结合蛋白, GRIM-19

Abstract: Objective To investigate the expressions of HBXIP and GRIM-19 in hepatocellular carcinoma tissues and their clinic significance. Methods Hepatocellular carcinoma tissue (n=42) and normal liver tissue (n=28) were collected from Tianjin First Central Hospital，immunohistochemistry was used to detect the expressions of HBXIP and GRIM-19 in these two groups. Results Rate of cells with positive expressions of HBXIP in hepatocellular carcinoma and normal liver tissues were 80.95%（34/42）and 42.86%（12/28）respectively; Rate of cells with positive expression of GRIM-19 in hepato⁃ cellular carcinoma tissues and normal liver tissues was 40.48%（17/42）and 75.00%（21/28）respectively, and the difference between these two groups was statistically significant（P < 0.05）; The expression of HBXIP was higher but the expression of GRIM-19 was lower in poor differentiated and stage Ⅲ-IV cells than those in well and moderate differentiated cells and in stage I-II, cells. What′s more, the expression of GRIM-19 is higher in tissue without portal thrombosis than that in tissue with portal thrombosis. The expression of HBXIP was negatively correlated with GRIM-19 expression（rS=-0.400，P < 0.01）. Conclusion The abnormal expressions of HBXIP and GRIM-19 may play important roles in the process of development and metastasis of hepatocellular carcinoma.

Key words: carcinoma, hepatocellular, neoplasm metastasis, immunohistochemistry, HBXIP, GRIM-19