天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (3): 330-335.doi: 10.11958/20150041

• 实验研究 • 上一篇    下一篇

黄连素抑制FSP27基因表达改善2型糖尿病地鼠内脏白色脂肪组织胰岛素抵抗的研究

李国生 ，刘栩晗， 李欣宇 ， 高政南 ， 黄澜 ， 刘亚莉   

  1. 1 大连医科大学附属第一医院病理科（邮编 116011）；2大连医科大学附属大连市中心医院内分泌科
  • 收稿日期:2015-07-14 修回日期:2015-11-20 出版日期:2016-03-15 发布日期:2016-03-15
  • 通讯作者: 刘栩晗 E-mail:guoshengli998@163.com
  • 作者简介: 李国生（1974）， 男， 副主任医师， 博士， 主要从事糖尿病病因及治疗机制研究

The investigation on the inhibitive effect of Berberine on gene expression of FSP27 to improve visceral white adipose tissues insulin resistance in type 2 diabetic hamsters

LI Guosheng, LIU Xuhan, LI Xinyu, GAO Zhengnan, HUANG Lan, LIU Yali   

  1. 1 Department of Pathology, The First Affiliated Hospital of Dalian Medical University, Liaoning 116011, China;2 Department of Endocrinology, Dalian Municipal Central Hospital of Dalian Medical University
  • Received:2015-07-14 Revised:2015-11-20 Published:2016-03-15 Online:2016-03-15
  • Contact: LIU Xuhan E-mail:guoshengli998@163.com

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摘要： 摘要：目的 研究黄连素（BBR）对2型糖尿病（T2DM）中国地鼠内脏白色脂肪组织（VWAT）中脂肪特异蛋白27（FSP27）和PR结构域蛋白16 （PRDM16）信号通路基因mRNA表达的影响并探讨相关机制。方法 以高脂饮食诱导肥胖胰岛素抵抗（OIR）地鼠模型，然后给予小剂量链脲菌素建立T2DM地鼠模型，对照组喂以普通饲料。造模完成后随机分成对照组、OIR组、肥胖T2DM组和T2DM BBR组。BBR治疗9周后，应用实时定量PCR方法检测各组地鼠VWAT中FSP27和PRDM16信号通路及其靶基因的mRNA表达改变。结果 与对照组相比，OIR组和肥胖T2DM组地鼠VWAT中PRDM16、CtBP-1、CtBP-2、C/EBPβ、PPARγ、PGC1α、PGC-1β及棕脂组织特异基因UCP-1、Cidea、Elovl3、PPARα及Acox、Cpt1和Acadm的mRNA表达降低，而FSP27和白脂组织特异基因Resistin、MEST和Serpina3k的mRNA表达增加。BBR治疗降低肥胖T2DM组地鼠VWAT中FSP27的表达而增强PRDM16信号通路效应，诱导棕脂组织特异基因mRNA的表达，诱导VWAT棕色化基因表型，改善脂诱性胰岛素抵抗。结论 BBR降低FSP27表达而增加PRDM16的表达与其诱导VWAT棕色化的分子机制相关，有助于增强产热耗能， 改善VWAT的异常脂代谢，改善FIVWATIR，恢复VWAT的功能。

关键词: 关键词：黄连素, 2型糖尿病, 白色脂肪组织棕色化, 脂肪组织, 胰岛素抵抗, 脂肪特异蛋白 27, PR 结构域蛋白 16

Abstract: Abstract: Objective To study the effects of berberine (BBR) on the gene mRNA expression of fat-specific protein 27 (FSP27) and PR domain containing 16 (PRDM16) signal pathway in visceral white adipose tissues (VWAT) from type 2 diabetic (T2DM) Chinese hamsters and explore the related mechanisms. Methods The obese insulin-resistant (OIR) hamster models were induced by high-fat diet and T2DM hamster models were created by OIR hamster models injected with low-dose streptozotocin, the control group hamsters were fed with standard laboratory chow. After the induction, the hamsters were randomly divided into control, OIR, obese T2DM and BBR-treated T2DM groups. After nine-week BBR treatment, real-time quantitative PCR was used to measure the gene mRNA expression changes of VWAT FSP27 and PRDM16 signal pathway and their target genes from different groups. Results Compared with control group, the gene mRNA expression of PRDM16, CtBP-1, CtBP-2, C/EBPβ, PPARγ, PGC1α, PGC-1β and brown adipose tissue-specific genes such as UCP-1, Cidea, Elovl3, PPARα, and Acox, Cpt1 and Acadm was decreased and that of FSP27 and white adipose tissue-specific genes including Resistin、MEST and Serpina3k was increased in VWAT from hamsters of OIR and obese T2DM groups. BBR treatment down-regulated FSP27 expression, enhanced PRDM16 signal pathway, and induced the gene mRNA expression of brown adipose tissue-specific genes in VWAT from obese T2DM group to develop browning gene phenotype of visceral white adipose tissues, and then improved fat-induced insulin resistance. Conclusion Decreased VWAT FSP27 expression and increased VWAT PRDM16 expression involved in the molecular mechanisms of browning of visceral white adipose tissues induced by BBR, and contributed to improve abnormal lipids metabolism and fat-induced insulin resistance in VWAT by enhancing consumption of energy as heat to restore VWAT function.

Key words: Key words: berberine, type 2 diabetes, browning of white adipose tissues, insulin resistance, FSP27