天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (3): 327-329.doi: 10.11958/20150032

• 实验研究 • 上一篇    下一篇

去肾交感神经术对犬心肌梗死后下丘脑血管紧张素II及氧化应激水平的影响

马艺杰1,卢成志1,张瑾2,王丽1,宋立君1,李超1   

  1. 1 天津医科大学一中心临床学院（邮编 300192）；2天津市第一中心医院心内科， 3 消化科
  • 收稿日期:2015-07-13 修回日期:2015-10-28 出版日期:2016-03-15 发布日期:2016-03-15
  • 通讯作者: 卢成志 E-mail:myjmessager@163.com
  • 作者简介:马艺杰（1990）， 女， 硕士在读， 主要从事高血压、冠心病研究
  • 基金资助:
    天津市应用基础与前沿技术研究计划;天津市卫生局科技基金项目

The effect of renal denervation on hypothalamus angiotensin II and oxidative stress in myocardial infarction dogs

MA Yijie, LU Chengzhi, LI Chao, ZHANG Jin, WANG Li, SONG Lijun   

  1. 1 First Center of Clinical College, Tianjin Medical University， Tianjin 300192, China; 2 Department of Cardiology, 3 Department of Gastroenterology, Tianjin First Center Hospital
  • Received:2015-07-13 Revised:2015-10-28 Published:2016-03-15 Online:2016-03-15
  • Contact: LU Chengzhi E-mail:myjmessager@163.com

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摘要： 摘要: 目的 探讨去肾交感神经术对心肌梗死(MI)犬下丘脑血管紧张素Ⅱ(AngⅡ)及氧化应激水平的影响。方法 18只犬随机分为MI组、MI+去肾交感神经术组（RDN组）和假手术组。前2组通过明胶海绵栓塞法建立MI模型，假手术组只行冠脉造影检查。MI后1周对RDN组行去肾交感神经术，余2组只行肾动脉造影检查。MI后4周检测AngⅡ、丙二醛(MDA)、超氧化物歧化酶(SOD)的含量及尼克酰胺腺嘌呤二核苷磷酸(NADPH)氧化酶亚基gp91phox蛋白的表达。结果 与假手术组相比，MI组下丘脑AngⅡ、MDA含量增加，gp91phox蛋白表达增加，SOD活性降低 (P<0.01)。MI组AngⅡ与SOD活性呈负相关，与gp91phox蛋白表达量呈正相关(rs 分别为-0.849和0.950, P<0.01)。RDN组较MI组AngⅡ、MDA含量降低，gp91phox蛋白表达降低，SOD活性增加 (P<0.01)。结论 去肾交感神经术可以降低下丘脑AngⅡ含量，抑制NADPH氧化酶表达，降低氧化应激水平，改善MI后心功能。

关键词: 心肌梗死, 下丘脑, 血管紧张素Ⅱ, 氧化性应激, 疾病模型, 动物, 去肾交感神经术

Abstract: Abstract: Objective To explore the effect of renal denervation (RDN) on hypothalamus angiotensin II and oxidative stress in myocardial infarction(MI) dogs. Methods Eighteen mongrel dogs were randomly divided into MI group (n=6), RDN group (n=6) and Sham operation group (n=6). Myocardial infarction model were made in the former two groups by gelatin sponge embolization of the left anterior descending artery. One week after MI, RDN was given to dogs in RDN group. Levels of hypothalamus angiotensin Ⅱ (AngⅡ), malondialdehyde (MDA), superoxide dismutase (SOD) and expression of gp91phox protein were detected four weeks after MI. Results Compared with control group, hypothalamus AngⅡ, MDA and expression of gp91phox protein were increased in MI group (P<0.01), but SOD was decreased (P<0.01). There was a positive correlation between AngⅡ and expression of gp91phox protein (rs =0.950, P<0.01) and negative correlation between AngⅡ and SOD (rs=-0.849, P<0.01) in MI group. Compared with MI group, hypothalamus AngⅡ, MDA and expression of gp91phox protein were decreased in RDN group (P<0.01),but SOD was increased (P<0.01).Conclusion RDN can decrease the level of hypothalamus AngⅡ and the level of hypothalamus oxidative stress , improve heart function of MI dogs.

Key words: myocardial infarction, hypothalamus, angiotensin Ⅱ, oxidative stress, disease models, animal, remove renal denervation surgery