天津医药 ›› 2016, Vol. 44 ›› Issue (6): 755-758.doi: 10.11958/20150262

• 临床研究 • 上一篇    下一篇

前列腺癌和前列腺增生中α辅肌动蛋白-1 的表达及其临床意义

李亚朋 1, 刘健 1, 曹凤宏 1, 李晓强 1, 陈宁 1, 2, 陈晨 1, 2   

  1. 1华北理工大学附属医院泌尿外科 (邮编 063000); 2华北理工大学
  • 收稿日期:2015-10-27 修回日期:2016-02-17 出版日期:2016-06-15 发布日期:2016-07-04

The expression and clinical significance of α-actinin-1 in prostate cancer and benign prostatic hyperplasia

LI Yapeng1, LIU Jian1, CAO Fenghong1, LI Xiaoqiang1, CHEN Ning1,2, CHEN Chen1,2   

  1. 1 Department of Urology, The Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, China;2 North China University of Science and Technology
  • Received:2015-10-27 Revised:2016-02-17 Published:2016-06-15 Online:2016-07-04

摘要: 摘要: 目的 探讨α辅肌动蛋白-1 (α-actinin-1, ACTN1) 在前列腺癌 (PCa) 和前列腺增生 (BPH) 中的表达及临床意义。方法 收集 2007 年 1 月—2014 年 10 月我校附属医院收治的 PCa 和 BPH 患者资料, 按一定的入组标准筛选, 采用免疫组织化学法检测 30 例 PCa 组织和 30 例 BPH 组织中 ACTN1 的表达情况, 采用蛋白免疫印迹(Western blot)方法检测其中的 18 例 PCa 组织和 20 例 BPH 组织中 ACTN1 的相对表达量。结果 免疫组织化学结果显示, ACTN1 在 PCa 和 BPH 两组中的阳性表达率分别为 76.7%和 20.0%, 差异有统计学意义 (P < 0.01); Western blot 结果显示, ACTN1 在 PCa 组中的相对表达量(0.591±0.182)明显高于 BPH 组(0.037±0.052), 差异有统计学意义(P < 0.05); 免疫组织化学结果中, ACTN1 在 PCa 中的表达在不同年龄、 血清前列腺特异抗原 (PSA) 水平、 是否有骨转移、是否有淋巴结转移患者之间差异无统计学意义 (P > 0.05), 而在不同 Gleason 分级、 T 分期患者之间差异有统计学意义(P < 0.05)。结论 ACTN1 在 PCa 组织中的表达明显高于 BPH 组织, 且在 PCa 组织中的表达与 Gleason 分级、 T 分期之间可能存在关联。

关键词: 前列腺肿瘤, 前列腺增生, 辅肌动蛋白, 免疫组织化学, 印迹法, 蛋白质, 前列腺癌, α辅肌动蛋白-1

Abstract: Abstract:Objective To investigate the expression and clinical significance of α- actinin- 1 protein (ACTN1) in prostate cancer (PCa) and prostatic hyperplasia (BPH). Methods The clinical data of patients with PCa or BPH treated in our school affiliated hospital were collected between January 2007—October 2014, according to certain criteria. Immunohistochemistry method was used to detect the expression of ACTN1 in 30 samples of PCa and 30 samples of BPH tissues. Western blot assay was used to detect the relative expression of ACTN1 in 18 samples of PCa and 20 samples of BPH tissues in two groups. Results The result of immunohistochemistry showed that the positive expression rates of ACTN1 were 76.7% and 20% in PCa and BPH groups respectively. The difference was statistically significant (P < 0.01). Western blot assay showed that the relative expression of ACTN1was significantly higher in PCa group (0.591±0.182) than that in BPH group (0.037 ± 0.052, P < 0.05). There was no significant difference in expression level of ACTN1 betweendifferent age groups. There was no significant difference in serum prostate specific antigen (PSA) level between patients with or without bone metastasis, and patients with or without lymph node metastasis (P > 0.05). There were significant differences in ACTN1 levels between different Gleason score and T staging groups (P < 0.05). Conclusion The expression of α-actinin-1 is significantly higher in PCa tissues than that in BPH tissues. There is the relationship between expression of ACTN1, Gleason scores and T staging.

Key words: prostatic neoplasms, prostatic hyperplasia, actinin, immunohistochemistry, blotting, Western, prostate cancer, α-actinin-1