天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (8): 989-992.doi: 10.11958/20150225

• 实验研究 • 上一篇    下一篇

自发性糖尿病GK 大鼠糖尿病周围神经病变模型的建立

富晓旭1 ， 冯露琳1 ， 张翕宇1 ， 胡芸2 ， 南小亚1 ， 谢春光1 ， 杜联3△   

  1. 1成都中医药大学临床医学院 （邮编610072）； 2重庆市中医院内分泌科； 3成都中医药大学基础医学院
  • 收稿日期:2015-10-14 修回日期:2016-03-07 出版日期:2016-08-15 发布日期:2016-08-22
  • 通讯作者: 杜联 E-mail:1325537604@qq.com E-mail:1325537604@qq.com
  • 作者简介: 富晓旭 （1988）， 女， 博士在读， 主要从事中医药防治内分泌及代谢性疾病的实验研究
  • 基金资助:
    国家自然科学基金资助项目 （81373783）

The establishment of diabetic peripheral neuropathy model in spontaneous diabetic GK rats

FU Xiaoxu1 ， Feng Lulin1 ， ZHANG Xiyu1 ， HU Yun2 ，NAN Xiaoya1 ， XIE Chunguang1 ， DU Lian3△   

  1. 1 Clinical Medicine College of Chengdu University of TCM, Chengdu 610072, China； 2 Department of Endocrinology, Chongqing TCM Hospital； 3 Basic Medical College of Chengdu University of TCM
  • Received:2015-10-14 Revised:2016-03-07 Published:2016-08-15 Online:2016-08-22
  • Contact: DU Lian E-mail:1325537604@qq.com E-mail:1325537604@qq.com

PDF (PC)

4131

摘要： 摘要： 目的 通过高脂饲养 GK 大鼠建立一种符合人类糖尿病周围神经病变 （DPN） 发病特点， 又操作简单的 DPN 大鼠模型。方法 7~8 周龄 SPF 级雄性自发性 2 型糖尿病 GK 大鼠 30 只， 喂养高脂饲料造模。另取 30 只 SPF 级正常 Wistar 大鼠作为正常组， 喂养普通饲料。每周监测动物血糖、 体质量、 饮水量、 饲料量。分别于干预 8 周、 12 周、 16 周后检测血清糖化血红蛋白、 坐骨神经传导速度， 取对侧坐骨神经做 HE 染色， TUNEL 染色计算细胞凋亡指数。结果 随着造模时间延长， GK 大鼠逐渐出现多饮、 多食、 生长迟缓等表现。与正常组相比， 造模 12 周及 16 周的大鼠血糖、 糖化血红蛋白升高 （P < 0.01）， 感觉神经传导速度降低 （P < 0.01）， 运动神经传导速度有一定下降趋势（12 周 P < 0.05， 16 周 P > 0.05）， 坐骨神经病理形态及雪旺细胞凋亡情况均提示 DPN 的形成， 并与正常大鼠形成鲜明对照 （凋亡指数 P < 0.01）。结论 长期高脂饲料喂养的 GK 大鼠是 DPN 研究的良好模型， 12 周是较为成熟且经济的造模时间。

关键词: 糖尿病神经病变, 疾病模型,动物, GK 大鼠

Abstract: Abstract： Objective To establish a simple diabetic peripheral neuropathy (DPN) rat model with the high fat-fed in GK rats. Methods A total of 30 GK rats (7-8 weeks) were fed with high-fat diet to establish the DPN model. Thirty normal Wistar rats were fed with ordinary diet (control group). The blood-sugar value, body mass, water-intake and food-intake were monitored every week in two groups. The serum level of glycosylated hemoglobin, the right sciatic nerve conduction velocity were detected at 8, 12 and 16 weeks respectively. The left sciatic nerve was used for HE and TUNEL staining. Results The manifestations of polydipsia, polyphagia and growth retardation were gradually appeared in GK rats. After 12 and 16 weeks, the blood-sugar and glycosylated hemoglobin were significantly increased in GK rats compared with those of normal Wistar rats (P < 0.01). The sensory nerve conduction velocity decreased obviously (P < 0.01). And motor nerve conduction velocity showed a certain decline trend (12 week P < 0.05， 16 week P > 0.05). The sciatic nerve pathological features and Schwann cell apoptosis suggested that the model of DPN was successfully established (apoptosis index, P < 0.01). Conclusion GK rats fed by high-fat diet are the satisfactory models of the DPN in experimental research. And 12- week is a suitable and economical time for molding.

Key words: diabetic neuropathies, disease models, animal, Goto-Kakizaki rats