天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (2): 210-212.doi: 10.11958/20150007

• 临床研究 • 上一篇    下一篇

Wnt2 与 Dvl 蛋白在食管鳞癌组织中的表达及临床意义

王勇涛， 王洪江△， 王兴名， 冯雪   

  1. 新疆医科大学附属肿瘤医院胸外科 （邮编830011）
  • 收稿日期:2015-07-03 修回日期:2015-10-26 出版日期:2016-02-15 发布日期:2016-02-15
  • 通讯作者: △通讯作者 E-mail： whj71210@sina.com E-mail:whj71210@sina.com
  • 基金资助:
    国家自然科学基金资助项目 （81160271）

Expression and clinical significance of Wnt2 and Dvl protein in esophageal aquamous carcinoma#br#

WANG Yongtao， WANG Hongjiang△， WANG Xingming， FENG Xue   

  1. Thoracic Surgery, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China
  • Received:2015-07-03 Revised:2015-10-26 Published:2016-02-15 Online:2016-02-15
  • Contact: △Corresponding Author E-mail: whj71210@sina.com E-mail:whj71210@sina.com

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摘要： 目的 检测 Wnt2 与散乱（Dvl）蛋白在食管鳞癌组织中的表达情况， 探讨其与食管鳞癌发生发展的关系。方法 采用 Western blot 法分别检测 Wnt2、 Dvl 蛋白在 60 例食管鳞癌组织及其对应癌旁组织中的表达， 并分析其与临床病理特征之间的关系。结果 Wnt2 和 Dvl 蛋白在食管鳞癌组织中的相对表达量 （0.512±0.406 和 1.218±1.082）高于癌旁食管组织 （0.153±0.189 和 0.505±0.358）， 且 Wnt2 及 Dvl 蛋白表达在不同浸润深度、 TNM 分期及淋巴结转移情况中存在差异（P＜0.01）； Wnt2 与 Dvl 蛋白在食管鳞癌组织中的表达呈正相关（r=0.718， P＜0.01）。结论 Wnt2、 Dvl 蛋白的高表达通过 Wnt2 信号转导通路协同促进食管鳞癌的发生、 发展及浸润转移。

关键词: 食管肿瘤, 癌, 鳞状细胞, Wnt2 蛋白质, Dvl 蛋白质, 印迹法, 蛋白质

Abstract: Objective To detect the expressions of Wnt2 and dishevelled (Dvl) protein in esophageal squamous carcinoma, and analyze their relationship with the occurrence and development of esophageal squamous cell carcinoma. Methods The expression levels of Wnt2 and Dvl protein were detected by Western blot assay in 60 samples of esophageal carcinoma and adjacent non-carcinomatous esophageal tissues, and their relationship with clinical pathological features were analyzed. Results The relative expression levels of Wnt2 and Dvl protein were higher in esophageal squamous carcinoma tissue (0.512 ± 0.406, 1.218±1.082) than those of esophageal tissue adjacent to carcinoma (0.153 ± 0.189, 0.505±0.358). There were significant differences in the expression levels of Wnt2 and Dvl protein between different infiltration depth, different TNM stages, and lymph node metastasis (P＜0.01). There was a positive correlation between Wnt2 and Dvl protein in esophageal squamous carcinoma (r = 0.718, P＜0.01). Conclusion The high expression levels of Wnt2 and Dvl protein promote the development and metastasis of esophageal squamous cell carcinomas collaboratively via Wnt2 signal transduction path⁃ ways.

Key words: esophageal neoplasms, carcinoma, squamous cell, Wnt2 protein, Dvl protein, blotting, Western