天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (9): 1164-1168.doi: 10.11958/20150392

• 流行病学调查 • 上一篇    下一篇

天津地区儿童急性呼吸道和消化道感染Saffold病毒的检出和临床流行特征

王维1,黄敬孚1,林书祥1,司萍1,李胜英1,祝益民2,杨洪江3   

  1. 1 天津市儿童医院， 天津市儿科研究所（邮编 300134）；2湖南省儿童医院急救中心；3 天津科技大学生物工程学院
  • 收稿日期:2015-12-14 修回日期:2016-05-09 出版日期:2016-09-15 发布日期:2016-09-28
  • 作者简介:王维（1980）， 女， 副研究员， 主要从事儿童病毒感染相关研究
  • 基金资助:

    国家十二五科技支撑计划（2012BAI04B01）；国家自然科学基金项目（31370205）；天津市卫生局科技基金（2012KY11）

Saffold virus detection and epidemiological characteristics in children with acute respiratory infection or digestive tract infection in Tianjin

WANG Wei1, HUANG Jingfu1, LIN Shuxiang1, SI Ping1, LI Shengying1, ZHU Yimin2, YANG Hongjiang3   

  1. 1 Pediatric Research Institute, Tianjin Children’s Hospital, Tianjin 300134, China; 2 Emergency Center, Hunan Children’s Hospital; 3 College of Life Science, Tianjin University of Science & Technology
  • Received:2015-12-14 Revised:2016-05-09 Published:2016-09-15 Online:2016-09-28

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摘要： 目的 调查天津地区儿童急性呼吸道和消化道感染中是否存在 Saffold 病毒（SAFV）感染及其流行病学特征。 方法 收集 360 份急性呼吸道感染患儿鼻咽抽吸物和 384 份消化道感染患儿粪便标本， 以针对 SAFV 5′-UTR 基因序列设计的特异性引物进行荧光定量 PCR 扩增， 随机取阳性扩增产物进行核苷酸序列测定， 并将所测序列在 GenBank 中进行比对。 计算呼吸道和消化道 SAFV 感染阳性者的阳性率， 不同年龄段及季节的 SAFV 阳性构成比和阳性检出率。 同时考察 SAFV 与其他病毒的混合感染情况。 结果 呼吸道和消化道检测 SAFV 阳性率分别为 11.9%（43/360）和 16.4%（63/384）。 不同性别的呼吸道 SAFV 阳性检出率差异无统计学意义［ 男 、女分别为 11.5% （28/243）和 12.8%（15/117）， χ2=0.13， P > 0.05］， 年龄 6 d～ 12 岁， ＜ 1 岁者占 79.0%（34/43）；消化道检测 SAFV 阳性患儿阳性检出率差异有统计学意义［ 男 、女分别为 13.4%（33/246）和 21.7%（30/138）， χ2=4.47， P＜ 0.05］， 年龄 5 h～ 11 岁。 不同年龄段的呼吸道和消化道 SAFV 阳性检出率差异均无统计学意义。 不同季节呼吸道 SAFV 阳性检出率差异有统计学意义， 以冬季和夏季为主（P＜ 0.01）， 而消化道 SAFV 阳性检出率差异无统计学意义。 呼吸道和消化道 SAFV 与其他病毒混合感染率分别为 7.0%（3/43）和 12.7%（8/63）。 结论 天津地区儿童存在 SAFV 感染， 在急性呼吸道和消化道感染患儿中具有较高的阳性检出率， 且在 1 岁以内患儿中的阳性检出率较高， 应该引起临床重视。

关键词: 呼吸道感染, 胃肠道, 流行病学研究, 天津, Saffold 心肌炎病毒, 儿童

Abstract: Objective To investigate prevalence and epidemiologic features of Saffold virus (SAFV) in hospitalized children with acute respiratory infection or digestive tract infection Tianjin area. Methods Nasopharyngeal aspirates from children with acute respiratory infection and fecal samples from children with digestive tract infection in Tianjin Children’ s Hospital were collected from January 2013 to December 2013. Viral nucleic acid was extracted, and SAFV infection was determined by using real-time quantitative PCR. Positive PCR products were sequenced. The sequencing results were aligned with known gene sequences of SAFV sequences in GenBank. The positive viral infection rate of nasopharyngeal aspirates and fecal samples, viral positive constituent ratio and positive detection rate in different age groups, seasonal distribution of SAFV infection were calculated. Other common respiratory tract or digestive tract viruses were also detected. Results Fourty-three (11.9 %) nasopharyngeal aspirates from children with acute respiratory infection tested positive for SAFV. There was no significant difference between male and female infected children (aged between 6 d and 12 years old). The 79 % (34/43) of the patients with SAFV infection aged under 1 year old. The infection most occurred in summer and winter. The 63 (16.4 %) fecal samples from children with digestive tract infection tested positive for SAFV. There was significant difference between male and female infected children (aged between 5 h and 11 years old). SAFV infection was found to be year round. There was no significant difference in different age groups of nasopharyngeal aspirates and fecal samples. The mixed infection rate with SAFV and other respiratory tract or digestive tract viruses were 7.0 %（3/43）and 12.7 %（8/63）, respectively. Conclusion Infection of SAFV had occurred in children with acute respiratory infection or digestive tract infection in Tianjin. SAFV has high detection rate in these children and is more common in children aged under 1 year old. The data suggest that some of acute respiratory infection or digestive tract infections in pediatric patients are related to SAFV. The Clinical doctors should pay attention to them ．

Key words: respiratory tract infections, gastrointestinal tract, epidemiologic studies, TIANJIN, Saffold virus, children