天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (10): 1251-1254.doi: 10.11958/20160518

• 临床研究 • 上一篇    下一篇

急性脑卒中患者外周血单个核细胞来源的AChE相关的microRNAs的表达变化

韩滨,马晓峰,张超   

  1. 天津医科大学总医院神经内科（邮编 300052）
  • 收稿日期:2016-06-06 修回日期:2016-07-06 出版日期:2016-10-15 发布日期:2016-10-21
  • 通讯作者: 张超 E-mail:zhangchao254@126.com
  • 作者简介:韩滨（1988）， 男， 硕士， 主要从事神经免疫性疾病的基础与临床研究
  • 基金资助:
    国家自然科学基金资助项目（81401361）

The change of AChE related microRNAs derived from peripheral blood mononuclear cells in acute ischemic stroke

HAN Bin, MA Xiaofeng, ZHANG Chao   

  1. Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China
  • Received:2016-06-06 Revised:2016-07-06 Published:2016-10-15 Online:2016-10-21
  • Contact: ZHANG Chao E-mail:zhangchao254@126.com

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摘要： 目的 探讨急性脑卒中患者外周血单个核细胞内乙酰胆碱酯酶（AChE）相关的 microRNAs 的表达变化。方法 利用 microRNAs 的预测软件并结合文献， 筛选靶向 AChE 的 microRNAs。 收集发病 24 h 以内的急性脑卒中患者和与之相匹配的对照组人群， 留取静脉血提取单个核细胞。 利用实时荧光定量 PCR（qRT-PCR） 技术检测 microRNAs 和 AChE mRNA 的表达， 利用 Western blot 技术检测 AChE 蛋白的表达。 结果 预测的靶向 AChE 的 microRNAs 包括 microRNA（miR）-24、-28、-124、-132、-182*、-194、-484。 与对照组相比， 脑卒中患者外周血单个核细胞内 miR-24、-124、-132 和-194 表达升高（P＜ 0.05）， miR-28、-182*及-484 无明显变化， AChE mRNA 和蛋白表达水平降低（P < 0.05）。 结论 脑卒中时 miRNAs 可能通过靶向 AChE 增强胆碱能抗炎通路。

关键词: 卒中, 乙酰胆碱酯酶, 微 RNAs, 外周血单个核细胞, 胆碱能抗炎通路

Abstract: Objective To investigate the expression changes of acetylcholinesterase (AChE) related microRNAs derived from peripheral blood mononuclear cells (PBMCs) in patients with stroke. Methods The microRNAs for targeting AChE mRNA were selected via prediction software and previous studies. PBMCs were extracted from venous blood samples of acute ischemic stroke patients (onset < 24 h) and healthy controls. The expressions of microRNAs and AChE mRNA were quantified using real-time PCR (RT-PCR). The protein level of AChE was detected by Western blot assay. Results The predicted microRNAs included microRNA (miR) - 24, -28, -124, -132, -182*, -194 and -484. The expression levels of miR-24, -124, -132 and-194 were significantly elevated in stroke patients compared with those of controls (P<0.05). There were no significant changes in expression levels of miR-28, -182* and -484. Additionally, the relative expression levels of intracellular AChE mRNA and protein decreased significantly in stroke patients (P<0.05). Conclusion MiRNAs can enhance cholinergic anti-inflammatory pathway by targeting AChE in patients with acute ischemic stroke.

Key words: stroke, acetylcholinesterase, microRNAs, peripheral blood mononuclear cells, cholinergic antiinflammatory pathwa