天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (1): 72-75.doi: 10.11958/20161119

• 临床研究 • 上一篇    下一篇

结肠癌患者血清中 microRNA-183 及胸苷激酶 1 的表达及意义

王金亮 1， 孟祥朝 2△， 张自立 2， 李琦 2 #br# #br#   

  1. 1 天津医科大学三中心临床学院 （邮编 300170）； 2 天津市第三中心医院
  • 收稿日期:2016-10-10 修回日期:2016-12-13 出版日期:2017-01-15 发布日期:2017-01-15
  • 通讯作者: 孟祥朝 E-mail:836319852wjl@sina.com
  • 基金资助:
    天津市卫计委重点攻关基金资助项目

The expression and significance of serum microRNA-183 and TK1 in patients with colorectal cancer

WANG Jin-liang1, MENG Xiang-chao2△, ZHANG Zi-li2, LI Qi2 #br# #br#   

  1. 1 The Third Central Clinical College of Tianjin Medical University, Tianjin 300170, China;
    2 Tianjin Third Central Hospital

  • Received:2016-10-10 Revised:2016-12-13 Published:2017-01-15 Online:2017-01-15

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摘要： 摘要:目的 检测结肠癌患者血清中 miRNA-183 及胸苷激酶 1 （TK1） 的表达并探讨其临床意义。方法 收集结 肠癌患者及健康体检者血清标本各 52 例， 荧光定量 PCR 检测 miRNA-183 表达， 免疫印迹增强化学发光法检测 TK1 的表达， 分析两者之间的相关性及与临床病理的关系。结果 与正常对照组相比， 结肠癌患者血清 miR-183 的表达 相对升高 （P < 0.01）； 中低分化腺癌者血清 miR-183 的表达量高于高分化腺癌 （P < 0.01）； Ⅲ～Ⅳ期结肠癌者血清中 miR-183 的表达量高于Ⅰ～Ⅱ期（P < 0.01）， 且在有淋巴结转移的患者中表达较高（P < 0.01）； 受试者工作特征 （ROC） 曲线显示， 以相对表达量 1.15 为临界点， 血清 miR-183 可能具有诊断结肠癌的价值， 其敏感度为 78.8%， 特异 度为 67.3%， 阳性预测值为 78.9%， 阴性预测值为 70.2%。结肠癌患者血清中 TK1 的表达也较正常对照组升高， 中低 分化腺癌高于高分化腺癌（P < 0.05）， Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期（P < 0.01）。血清中 miR-183 与 TK1 的表达呈正相关 （rs=0.692， P＜0.01）。结论 血清中 miR-183 及 TK1 可能是早期结肠癌的诊断标志物， 并可用于判断肿瘤的恶性程 度及预后。

关键词: 结肠肿瘤, 微 RNAs, 胸苷激酶, 血清, 结肠癌, miRNA-183

Abstract: Abstract: Objective To detect the expression and significance of serum miRNA- 183 and TK1 in patients with colorectal cancer, and the mechanism thereof. Methods Fifty-two serum samples of colorectal cancer patients and paired health serum samples were collected. The expression of miRNA-183 was detected by real-time quantitative PCR, and TK1 was detected by Western blot enhanced chemiluminescence assay. The correlation between miRNA-183 and TK1 and their relations with the clinicpathologic characteristics were analyzed. Results The serum miRNA- 183 expression was significantly higher in colorectal cancer group than that in normal control group (P < 0.01). The expression of serum miRNA- 183 was significantly higher in stage Ⅲ-Ⅳ group than that in stage Ⅰ-Ⅱ group (P < 0.01). There was more significant increase in serum miRNA- 183 in lymphatic metastasis group than that without lymphatic metastasis group (P < 0.01). Receiver operating characteristic (ROC) curve showed that of there was a diagnostic value for serum miRNA- 183 in colorectal cancer, with an optimal value of 1.15. The diagnostic sensitivity and specificity were 78.8% and 67.3%, and the positive predictive value and negative predictive value were 78.9% and 70.2%. The serum TK1 expression was also higher in colorectal cancer group compared with that of normal control group (P < 0.01). And the TK1 expression was also higher in stage Ⅲ-Ⅳ group than that in stage Ⅰ-Ⅱ group (P < 0.01). Furthermore, miRNA-183 expression was positively correlated with TK1 expression (rs=0.692, P < 0.01). Conclusion The serum expression levels of miR-183 and TK1 may act as tumor markers for early colorectal cancer diagnosis, and also can be used to predict the malignant degree and prognosis of colorectal cancer.

Key words: colonic neoplasms, microRNAs, thymidine kinase, serum, colon cancer, miR-183