天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (7): 735-738.doi: 10.11958/20170332

• 临床研究 • 上一篇    下一篇

Syntaxin8 在非小细胞肺癌组织中的表达及临床意义

孙金鹏，田涛，陈玲，刘燕春， 孔继昌   

  1. 1 河北省沧州中西医结合医院 （邮编 061000）； 2 华北石油管理局总医院； 3 沧州市医学高等专科学校
  • 收稿日期:2017-03-15 修回日期:2017-05-12 出版日期:2017-07-15 发布日期:2017-08-08

Research of the expression and significance of syntaxin 8 in non-small cell lung cancer tissues

SUN Jin-peng,TIAN Tao,CHEN Ling,LIU Yan-chun,KONG Ji-chang   

  1. 1 Cangzhou Hospital of Integrated Traditional and Western Medicine, Hebei 061000, China; 2 General Hospital of North China Petroleum Administration Bureau;3 Cangzhou Medical College
  • Received:2017-03-15 Revised:2017-05-12 Published:2017-07-15 Online:2017-08-08

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摘要： 目的 检测突触融合蛋白 8（Syntaxin 8， STX8）在非小细胞肺癌（NSCLC）组织中的表达， 分析其表达与NSCLC 临床病理特征的关系。方法 收集 NSCLC 癌组织及癌旁组织各 70 例用于免疫组化染色， 并另取 NSCLC 组织及癌旁组织各 10 例用于 RNA 和蛋白提取， 采用 qRT-PCR 检测 STX8 mRNA 相对表达量， 采用 Western blot 法检测 STX8 蛋白表达， 分析 STX8 的表达与 NSCLC 临床病理特征的关系。结果 NSCLC 组织中 STX8 mRNA 表达水平高于癌旁组织 （3.962 5±0.487 3 vs. 0.538 2±0.097 5， t=21.797， P<0.01）； 免疫组化结果显示， STX8 蛋白在 NSCLC 组织中高表达率高于癌旁组织［71.43%（50/70）vs. 38.57%（27/70）， χ2=15.267， P<0.01］； 并且 NSCLC 组织中 STX8 蛋白表达水平同样高于癌旁组织（2.496 1±0.362 5 vs. 0.340 2±0.119 1， t=17.876， P<0.01）； STX8 在不同的病理分型 NSCLC患者癌组织中高表达率不同 （P<0.05）， 在鳞状细胞癌、 腺癌中高表达率高于腺鳞癌， 大细胞癌中没有观察到 STX8 高表达； 不同性别、 年龄、 肿瘤直径、 TNM 分期、 有无淋巴结转移分组的 NSCLC 患者癌组织中 STX8 表达差异无统计学意义。结论 STX8 在 NSCLC 中异常高表达， 可能与 NSCLC 的发生发展进程相关。

关键词: 癌, 非小细胞肺, SNARE 蛋白质类, 突触融合蛋白 8

Abstract: Objective To investigate the expression and significance of syntaxin 8 (STX8) in non- small cell lung cancer (NSCLC), and analyze the relationship between STX8 expression and clinicopathological features of NSCLC.Methods Seventy samples of NSCLC and 70 samples of pericancerous tissues were collected for immunohistochemistry staining, and another 10 samples of NSCLC and pericancerous tissues were used for RNA and protein extraction. qRT-PCR was performed to detect the expression of STX8 mRNA, and Western blot assay was adopted to detect the expression of STX8 protein. The relationship between STX8 expression and clinicopathological features of NSCLC was analyzed. Results Results of qRT-PCR showed that the expression of STX8 mRNA was significantly higher in NSCLC tissues than that in the pericancerous tissues (3.962 5±0. 487 3 vs. 0.538 2±0.097 5, t=21.797, P<0.01). Immunohistochemistry results showed that the high expression rate of STX8 was significantly higher in NSCLC tissues than that in the pericancerous tissues [71.43%(50/70) vs. 38.57% (27/70), χ2=15.267, P<0.01]. Western blot results showed that the expression of STX8 protein was significantly higher in NSCLC tissues than that in the pericancerous tissues (2.496 1±0.362 5 vs. 0.340 2±0.119 1, t=17.876,P<0.01). The high expression rate of STX8 was significantly different in different histological types of NSCLC (P <0.05). The high expression rate of STX8 was higher in squamous cell carcinoma and adenocarcinoma than that in adenosquamous carcinoma, and the high expression rate of STX8 was not observed in large cell carcinoma. There were no significant differences in expressions of STX8 in NSCLC patients with different gender, ages, tumor diameters, TNM stages and with or without lymph node metastasis (P>0.05). Conclusion A high expression of STX8 in NSCLC tissues may be correlated with the occurrence and development of NSCLC.

Key words: carcinoma, non-small-cell lung, SNARE proteins, syntaxin 8

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