天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (10): 1013-1016.doi: 10.11958/20170719

• 细胞与分子生物学 • 上一篇    下一篇

microRNA-155 通过 MyD88 抑制泡沫细胞免疫炎症反应

荆祥阳，刘运龄，路利平，梁雪，刘恩照   

  1. 天津医科大学第二医院心脏科，天津市心血管病离子与分子机能重点实验室，天津心脏病学研究所（邮编 300211）
  • 收稿日期:2017-06-21 修回日期:2017-07-08 出版日期:2017-10-15 发布日期:2017-10-13
  • 通讯作者: 荆祥阳 E-mail:jingxystu@163.com
  • 作者简介:荆祥阳（1992），男，硕士在读，主要从事冠心病和心律失常的临床与基础研究

microRNA-155 inhibits immuno-inflammatory reaction of foam cells by targeting MyD88

JING Xiang-yang, LIU Yun-ling, LU Li-ping, LIANG Xue, LIU En-zhao   

  1. Department of Cardiology, the Second Hospital of Tianjin Medical University, Tianjin Key Laboratory of Ionic- Molecular Function of Cardiovascular Disease, Tianjin Institute of Cardiology, Tianjin 300211, China
  • Received:2017-06-21 Revised:2017-07-08 Published:2017-10-15 Online:2017-10-13
  • Contact: Xiang-Yang JING E-mail:jingxystu@163.com

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摘要： 目的 探讨 microRNA-155（miR-155）通过髓样分化因子 88（MyD88）对泡沫细胞免疫炎症反应的影响及 其可能机制。方法 体外培养小鼠单核巨噬细胞系 RAW264.7，分别转染 miR-155 mimics、miR-155 inhibitor 和 MyD88 siRNA 及其阴性对照组，并用氧化低密度脂蛋白（ox-LDL）刺激形成泡沫细胞，采用 RT-qPCR 和 Western blot 检测 MyD88 mRNA 和蛋白的表达，采用 ELISA 法检测细胞上清液中炎症因子白细胞介素（IL）-10、转化生长因子 （TGF）-β1 和单核细胞趋化蛋白-1（MCP-1）的含量。结果 与阴性对照组相比，转染 miR-155 mimics 后泡沫细胞 中 MyD88 mRNA 表达未见明显变化（P＞0.05），而 MyD88 蛋白表达和炎症因子 IL-10、TGF-β1 和 MCP-1 的表达水 平明显降低（P＜0.05）；转染 miR-155 inhibitor 后泡沫细胞中 MyD88 mRNA 表达无明显变化（P＞0.05），MyD88 蛋白 和炎症因子的表达水平显著上调（P＜0.05）；转染 MyD88 siRNA 后泡沫细胞中 MyD88 mRNA 和蛋白表达水平明显 下调，炎症因子的分泌也随之降低（P＜0.05）。结论 miR-155 可在翻译水平靶向调控 MyD88 的表达，从而抑制免 疫炎症反应。

关键词: 微 RNAs, 动脉粥样硬化, 髓样分化因子 88, 泡沫细胞, miR-155, 氧化低密度脂蛋白, 炎症因子

Abstract: Objective To investigate the effects of microRNA- 155 (miR-155) on immuno-inflammatory reaction of foam cells by targeting MyD88 and the possible mechanism. Methods RAW264.7 macrophages were cultured in vitro and transfected with miR-155 mimics, miR-155 inhibitor, MyD88 siRNA and their negative control respectively, then ox-LDL stimulation was given to build foam cell model. The expression of MyD88 in foam cells was detected by RT- qPCR and Western blot assay. Moreover, the expression levels of interleukin (IL)- 10, TGF- β1 and MCP- 1 in supernatant were determined by ELISA. Results After being transfected with miR- 155 mimics, the mRNA level of MyD88 remained unchanged compared with that of control group (P＞0.05). The protein level of MyD88 decreased significantly (P＜0.05), and the expression levels of IL-10, TGF-β1 and MCP-1 in supernatant also decreased significantly (P＜0.05). After being transfected with miR- 155 inhibitor, the mRNA level of MyD88 remained unchanged compared with that of control group (P＞0.05). The expression levels of MyD88 protein and inflammatory cytokines increased significantly (P＜0.05). After being transfected with MyD88 siRNA, the expression levels of MyD88 mRNA and protein decreased significantly, and the expression levels of inflammatory cytokines also decreased significantly (P＜0.05). Conclusion miR-155 can negatively regulate inflammation by targeting MyD88 through the inhibition of translation.

Key words: microRNAs, atherosclerosis, myeloid differentiation factor 88, foam cells, miR- 155, ox- LDL, inflammatory factor