天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (2): 136-139.doi: 10.11958/20181351

• 实验研究 • 上一篇    下一篇

JAK2/STAT3信号转导通路在子宫内膜异位症发病过程中的作用

陈建梅1 ， 杨蕊蕊1 ， 刘洪恩1 ， 刘洪欣2 ， 史文新3 ， 刘姣3， 4△   

  1.  1山东省滨州市人民医院妇科 （邮编256600）； 2滨州医学院附属医院心内科； 3河北医科大学生理教研室； 4河北中医学院中药药理教研室
  • 收稿日期:2018-09-06 修回日期:2018-12-15 出版日期:2019-02-15 发布日期:2019-02-15
  • 通讯作者: 刘姣 E-mail:fairypc_lj@163.com
  • 基金资助:
    三棱-莪术通过调控FABP4表达干预子宫内膜异位症的配伍机制研究

The role of JAK2/STAT3 signal transduction pathway in the pathogenesis of endometriosis

CHEN Jian-mei 1 , YANG Rui-rui 1 , LIU Hong-en1 , LIU Hong-xin2 , SHI Wen-xin3 , LIU Jiao3,4△   

  1. 1 Department of Gynecology, Binzhou People's Hospital, Binzhou 256600, China; 2 Department of Cardiology, Binzhou Medical University Hospital; 3 Department of Physiology, Hebei Medical University; 4 Chinese Medicine Pharmacology Teaching and Research Office, Hebei University of Chinese Medicine
  • Received:2018-09-06 Revised:2018-12-15 Published:2019-02-15 Online:2019-02-15

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摘要： 目的 研究JAK2/STAT3信号转导通路在子宫内膜异位症 （EMS） 发展过程中的作用。方法 将60只大鼠随机分为假手术组、 模型组、 JAK2/STAT3通路抑制剂组 （AG组）， 每组20只。模型组、 AG组大鼠通过自身子宫内膜移植建立EMS模型。AG组造模前给予AG490 1 mg/kg， 手术后给予AG490 4 mg/kg， 每周2次， 连续4周； 模型组给予生理盐水。处理结束后记录各组大鼠异位内膜体积， 计算异位内膜生长抑制率， HE染色观察病理改变； 蛋白质印迹法 （Western blot） 检测内膜组织中p-JAK2、 JAK2、 p-STAT3、 STAT3蛋白表达水平。结果 治疗后AG组异位内膜组织生长发育不良， 异位内膜体积明显小于模型组， 异位内膜生长的抑制率为65.66%。Western blot结果显示， EMS模型大鼠异位内膜病灶中JAK2、 STAT3总蛋白及其磷酸化水平 （p-JAK2/JAK2、 p-STAT3/STAT3） 明显高于假手术组； 而 AG组大鼠异位内膜病灶中JAK2、 STAT3总蛋白及其磷酸化水平明显低于模型组。结论 JAK2/STAT3通路在EMS 发病过程中可能发挥了重要作用， 而抑制JAK2/STAT3信号通路则能够对EMS的治疗产生积极作用。

关键词:  子宫内膜异位症, Janus激酶2, STAT3转录因子, 信号传导, AG490

Abstract: Objective To analyze the relationship between the JAK2 / STAT3 signal transduction pathway and endometriosis (EMS). Methods Sixty rats were randomly divided into Sham-operated group, model group and JAK2 / STAT3 pathway inhibitor group (AG group). There were 20 rats in each group. In model group and AG group, EMS model was established by endometrial transplantation. The rats in AG group were given AG490 1 mg/kg 5 min before modeling, and AG490 4 mg/kg after surgery, 2 times a week for 4 weeks. The model group was given same volume of normal saline. After treatment, the volume of ectopic endometrium was recorded, the growth inhibition rate of ectopic endometrium was calculated, and the histopathological changes were observed by HE staining. The protein expression levels of p-JAK2, JAK2, p-STAT3 and STAT3 were detected by Western blot assay. Results After treatment, the volume of ectopic endometrium was significantly smaller in AG group than that in model group, and the inhibition rate of ectopic endometrial growth was 65.66%. The results of Western blot assay showed that JAK2 and STAT3 proteins and their phosphorylation levels (p-JAK2/ JAK2 and p-STAT3 / STAT3) were significantly increased in ectopic lesions of EMS rats compared with those in normal uterus. The expression levels of p-JAK2, p-STAT3, JAK2 and STAT3 were significantly decreased in ectopic lesions of AG group rats compared with those in EMS rats. Conclusion The JAK2/STAT3 pathway may play an important role in the pathogenesis of EMS, and the inhibition of JAK2/STAT3 signaling pathway can play a positive role in the treatment of EMS.

Key words: endometriosis, Janus kinase 2, STAT3 transcription factor, signal transduction, AG490