天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (1): 19-22.doi: 10.11958/20181383

• 实验研究 • 上一篇    下一篇

艾灸对缺氧缺血性脑病新生小鼠脑组织炎症细胞浸润和 CD11b表达的影响

肖爱娇 1，肖移生 1，欧阳昕 2，贺琳 1，陈明人 2△   

  1. 基金项目：国家自然科学基金资助项目（81660819）；江西省科技厅重点研发计划项目（20161BBH80053）；江西省卫生计生委中医药科研 计划课题（2018A369）；江西中医药大学科研项目（2014ZR018，2015jzzdxk024） 作者单位：1江西中医药大学基础医学院（邮编330004），2灸学院 作者简介：肖爱娇（1973），女，博士，教授，主要从事中医药神经保护作用机制研究 △通讯作者 E-mail：chenmr928@163.com
  • 收稿日期:2018-09-11 修回日期:2018-11-29 出版日期:2019-01-15 发布日期:2019-01-15
  • 通讯作者: 肖爱娇 E-mail:xaj527@163.com
  • 基金资助:
    艾灸减轻新生小鼠缺氧缺血性脑损伤的抗线粒体凋亡信号通路研究

Effects of moxibustion on inflammatory cell infiltration and CD11b expression in brain tissue of neonatal mice with hypoxic-ischemic encephalopathy

XIAO Ai-jiao1, XIAO Yi-sheng1, OU-YANG Xin2, HE Lin1,.CHEN Ming-ren2△   

  1. 1 College of Basic Medicine Science, 2 College of Moxibustion, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China △Corresponding Author E-mail：chenmr928@163.com
  • Received:2018-09-11 Revised:2018-11-29 Published:2019-01-15 Online:2019-01-15

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摘要： 目的 观察艾灸对缺氧缺血性脑病新生小鼠脑组织炎症细胞浸润和CD11b表达的影响。方法 出生7 d 新生ICR小鼠，随机分为3组：假手术组（n=20）、模型组（n=24）和艾灸组（n=20）。假手术组只做颈部手术，不闭合颈 总动脉，不缺氧；模型组行颈总动脉闭合术，并给予缺氧处理；艾灸组在模型组的基础上给予艾灸治疗，每日1次，35 min/次。采用TTC染色检测小鼠脑梗死的面积；HE染色观察小鼠脑组织形态结构和炎症细胞浸润；组织免疫荧光染 色检测脑组织CD11b表达。结果 与假手术组相比，模型组小鼠的脑梗死面积增大，患侧脑组织大量细胞坏死脱 落，并伴有大量炎症细胞浸润，CD11b阳性细胞数明显增多；与模型组相比，艾灸组小鼠的脑梗死面积缩小，患侧脑组 织细胞排列较致密、整齐，炎症细胞较少，CD11b阳性细胞数明显减少。结论 艾灸具有减轻新生小鼠缺氧缺血性脑 损伤的作用，这可能与其减少脑组织小胶质细胞浸润、减轻炎症反应有关。

关键词: 抗原, CD11b, 缺氧缺血, 脑, 动物, 新生, 艾灸治疗

Abstract: Objective To investigate the effects of moxibustion on inflammatory cell infiltration and CD11b expression in ipsilateral brain of a neonatal mouse model with hypoxic-ischemic (HI) brain injury. Methods Postnatal 7 d ICR mice were randomly divided into three groups: sham group (n=20), HI group (n=24) and moxibustion-treated group (n=20). For sham animals, only neck surgery was performed and the common carotid artery was not occluded and was not exposed to hypoxia. For HI animals, the common carotid artery was occluded and exposed to hypoxia, while for moxibustion-treated animals, moxibustion treatment (once a day for 35 minutes) was applied in addition to HI procedure. TTC staining was used to examine area of cerebral infarction in rats, HE staining was used to detect inflammatory cells, and immunofluorescent staining was used to examine the CD11b-positive cells in the ipsilateral brain. Results Mice in HI group showed larger infarct area, more cell loss, more inflammatory cells and more CD11b-positive cells in the ipsilateral brain than those in sham group, while mice in moxibuston-treated group showed smaller infarct area, less cell loss, less inflammatory cells and less CD11b -positive cells in the ipsilateral brain than those in HI group. Conclusion Moxibustion can decrease microglia infiltration and alleviate inflammation reaction, hence reducing brain injury after HI procedure.

Key words: antigens, CD11b, hypoxia-ischemia, brain, animals, newborn, moxibustion treatment