天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (5): 421-426.doi: 10.11958/20192651

• 临床研究 • 上一篇    下一篇

Reg Ⅳ在不同分子分型乳腺癌中的表达及意义 #br#

宋姗姗 1，聂艳红 1，张春芳 1，林海月 1，张强 2，陈昊 1△
  

  1. 1徐州医科大学附属连云港市第一人民医院病理科（邮编 222061）；2 连云港市第二人民医院胃肠外科

  • 收稿日期:2019-08-27 修回日期:2020-03-04 出版日期:2020-05-15 发布日期:2020-06-24
  • 通讯作者: 陈昊 E-mail:E-mail: lygbingli@sina.com

Expression and significance of Reg Ⅳ in breast cancer and its specific molecular subtypes #br#

SONG Shan-shan1, NIE Yan-hong1, ZHANG Chun-fang1, LIN Hai-Yue1, ZHANG Qiang2, CHEN Hao1△ #br#   

  1. 1 Department of Pathology, the First People’s Hospital of Lianyungang, the Affiliated Hospital of Xuzhou Medical University,
    Jiangsu 222061, China; 2 Department of Gastrointestinal Surgery, the Second People's Hospital of Lianyungang
  • Received:2019-08-27 Revised:2020-03-04 Published:2020-05-15 Online:2020-06-24

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摘要： 目的 检测再生基因衍生家族成员IV（regenerating islet-derived family, member 4, Reg IV）在恶性乳腺癌及其不同分子分型中的表达及临床意义。方法 收集140例恶性乳腺癌手术切除标本，其中LA型30例、LB型50例、HER-2+30例、TNBC型30例。以其中75例癌旁的正常乳腺组织作为对照，采用EnVison免疫组化、实时荧光定量PCR法和生物数据库分析Reg IV蛋白和基因在恶性乳腺癌及不同分子分型中的表达情况。结果 （1）Reg IV蛋白在正常组织中的阳性率（25.33%）低于癌组织(49.29%)，在非TNBC中的阳性率（56.36%）高于TNBC型（23.33%），差异有统计学意义(P＜0.05)；（2）Reg IVmRNA在恶性乳腺癌中的表达量（2.040.66）高于正常乳腺组织（1.190.47），且在TNBC型中的表达量均低于非TNBC型乳腺癌（P＜0.05）；（3）Ualcan数据库中Reg IVmRNA在乳腺癌中的表达量高于正常组织，差异有统计学意义（P＜0.05），在非TNBC型中的表达较TNBC的表达水平有增高趋势，但无统计学差异（P≥0.05）,GCBI数据库中，Reg IV在乳腺癌中的表达量亦显著高于正常乳腺组织 ；(4) Reg IV在ER、PR和HER-2阳性组中的表达明显高于阴性组，而与EGFR和Ki-67表达无关；（5）Reg IV的表达与患者年龄、肿瘤直径、TNM分期及预后相关（P＜0.05），而与淋巴结转移、组织学分级和部位无关。结论 Reg IV在乳腺癌，尤其非TNBC型的发生发展中可能发挥作用，且与患者的预后相关，有望为乳腺癌的治疗提供潜在的治疗靶点。

关键词: 乳腺肿瘤, 癌, 再生胰岛衍生家族成员Ⅳ, 预后, 分子分型, 临床病理特征

Abstract: Objective We aimed to investigate the expression of Reg IV in breast cancer and specific molecular subtypes. The relationships between Reg IV and clinical pathological features were also analyzed. Methods 75 cases of normal breast tissues and 140 cases of malignant breast cancer, which including 30 cases of LA type, 50 cases of LB type, 30 cases of HER-2+ type, and 30 cases of TNBC type, were involved in the current study. EnVison immunohistochemistry was used to detect the expression of Reg IV. Real-time quantitative PCR and biological databases were used to analyze Reg IV protein mRNA and the association with clinical pathological features. Results The expression of Reg IV mRNA and protein was higher in malignant breast cancer compared with normal breast tissues (P＜0.05). The level of Reg IV in non TNBC was higher than that in TNBC (P＜0.05). Additionally, Reg IV up-regulation was related to positive ER, PR and HER-2 (P＜0.05), instead of Ki-67 and EGFR. Reg IV was also significantly associated with age, tumor size, TNM stage and prognosis (P＜0.05), but no significantly correlated with lymph node metastasis, histological grade and position. Conclusion Our research demonstrated that Reg IV may play an important role in malignant breast cancer, especially in non TNBC. Reg IV may be a promising biomarker for the prognosis of breast cancer patients.

Key words: breast neoplasms；carcinoma；regenerating islet-derived family member Ⅳ；prognosis, molecular subtypes；
clinical pathological features

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