天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (11): 1074-1078.doi: 10.11958/20200336

• 临床研究 • 上一篇    下一篇

茶多酚对早期龋进展的影响

马丽1，陈静1，郭爱花2，张向宇1△   

  1. 1天津医科大学口腔医院口腔预防科及儿童口腔科（邮编300070），2口腔种植科
  • 收稿日期:2020-03-02 修回日期:2020-08-24 出版日期:2020-11-15 发布日期:2020-11-15

The effect of tea polyphenols on early caries progression

MA Li1, CHEN Jing1, GUO Ai-hua2, ZHANG Xiang-yu1△   

  1. 1 Department of Preventive and Pediatric Dentistry, 2 Department of Dental Implantation, Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China
  • Received:2020-03-02 Revised:2020-08-24 Published:2020-11-15 Online:2020-11-15

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摘要： 目的　探讨茶多酚对牙齿早期龋进展的影响。方法　采用体外脱矿的方法建立牛牙牙釉质和牙本质早期龋模型。将釉质龋及牙本质龋标本随机分为4组（n=6）：空白对照组（DW组）、氟化钠组（NaF组）、茶多酚组（TP组）、茶多酚与氟化钠联合应用组（TP+NaF组），pH循环实验模拟早期龋的进程。检测pH循环后的脱矿液中钙、磷离子浓度，通过显微硬度计检测牙体硬组织表面显微硬度值，X线能谱分析样本表面钙磷比，并测定再矿化液中羟脯氨酸水平。结果　在牙釉质及牙本质的脱矿液中，TP组钙、磷离子相对释放量与DW组差异无统计学意义，明显高于NaF组和TP+NaF组（P＜0.05）。TP组牙釉质表面显微硬度值与DW组差异无统计学意义，牙本质表面显微硬度值高于DW组；DW组、TP组牙釉质及牙本质表面显微硬度值均低于NaF组和TP+NaF组（P＜0.05）。TP组牙釉质及牙本质表面钙磷比均高于DW组（P＜0.05），牙釉质表面钙磷比与NaF组和TP+NaF组差异无统计学意义，牙本质表面钙磷比低于NaF组和TP+NaF组（P＜0.05）。NaF组和TP+NaF组牙本质再矿化液中羟脯氨酸水平均低于DW组和TP组（P＜0.05），TP组与DW组以及TP+NaF组与NaF组间差异均无统计学意义。结论　在早期龋的进展过程中，茶多酚无明显抑制牙釉质及牙本质脱矿的作用，但可通过促进钙磷离子的再沉积促进再矿化的发生。

关键词: 龋齿, 多酚类, 氟化钠, 茶多酚, 脱矿, 再矿化

Abstract: Objective To investigate the effect of tea polyphenols on early dental caries progression. Methods An early caries model of enamel and dentin of bovine teeth was established by extracorporeal demineralization. The samples of enamel caries and dentin caries were randomly divided into 4 groups (n=6): blank control group (DW group), sodium fluoride group (NaF group), tea polyphenol group (TP group) and tea polyphenols combined with sodium fluoride group (TP+NaF group). The pH circulation experiment was conducted to simulate the process of early caries. The concentrations of calcium and phosphate ions in the demineralization solution after pH cycle were detected, and the microhardness of tooth surface was detected by surface microhardness tester. X-ray energy spectrum was used to analyze calcium-phosphate ratio on tooth surface, and the hydroxyproline level in the remineralization solution was determined. Results There was no significant difference in the release of calcium and phosphate ions of the demineralization solution of enamel and dentin between TP group and the DW group, which was significantly higher than that of NaF group and TP+NaF group (P＜0.05). There was no significant difference in the microhardness of enamel surface between TP group and DW group, and the microhardness of dentin surface was higher than that of DW group. The microhardness of enamel and dentin surface was lower in DW group and TP group than that of NaF group and TP+NaF group (P＜0.05). Calcium-phosphate ratio of enamel and dentin surface was significantly higher in TP group than that in DW group (P＜0.05). There was no significant difference in the calcium-phosphate ratio of enamel surface between TP group, NaF group and TP+NaF group, while the calcium-phosphate ratio of dentin surface was significantly lower in TP group than that of the NaF group and TP+NaF group (P＜0.05). The levels of hydroxyproline in dentin remineralization solution were significantly lower in NaF group and TP+NaF group than those of DW group and TP group (P＜0.05). There were no significant difference between TP group and DW group, and TP+NaFgroup and NaF group. Conclusion During the early caries progression, tea polyphenols dose not significantly inhibit enamel and dentin demineralization, but could promote the redeposition of calcium and phosphate ions, which can effectively delay the development of dental caries.

Key words: dental caries, polyphenols, sodium fluoride, tea polyphenols, demineralization, remineralization