天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (9): 834-838.doi: 10.11958/20201173

• 实验研究 • 上一篇    下一篇

加味大柴胡汤对梗阻性黄疸大鼠肝损伤及JNK、Bcl-2表达的影响#br#

肖黄满1，尚海涛2，张静宇2，郝成飞2，刘军舰2，陈帅1，张景虹1，李忠廉2△   

  1. 1天津医科大学研究生院（邮编300070）；2天津市南开医院肝胆胰第二外科
  • 收稿日期:2020-04-29 修回日期:2020-06-18 出版日期:2020-09-15 发布日期:2020-09-15
  • 通讯作者: 李忠廉 E-mail:nkyylzl@163.com
  • 作者简介:肖黄满（1992），男，硕士在读，主要从事肝胆胰外科的基础与临床研究
  • 基金资助:
    国家自然科学基金面上项目（81273952）；天津市中医药管理局一般项目（2019003）

Effects of Jiawei Dachaihu decoction on liver injury and expressions of JNK and Bcl-2 proteins in obstructive jaundice rats#br#

XIAO Huang-man1, SHANG Hai-tao2, ZHANG Jing-yu2, HAO Cheng-fei2, LIU Jun-jian2, CHEN Shuai1, ZHANG Jing-hong1, LI Zhong-lian2∆#br#   

  1. 1 Graduate School of Tianjin Medical University, Tianjin 300070, China; 2 Department of the Second 
    Hepatobiliary and Pancreatic Surgery, Tianjin Nankai Hospital
  • Received:2020-04-29 Revised:2020-06-18 Published:2020-09-15 Online:2020-09-15

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摘要： 摘要：目的　探讨加味大柴胡汤对梗阻性黄疸大鼠肝损伤及JNK、Bcl-2 mRNA和蛋白表达的影响。方法　将90只SD雄性大鼠按随机数表法分为假手术组（S组）、梗阻性黄疸组（O组）及干预组（M组），每组30只。S组与O组每日定时生理盐水10 µL/g灌胃，M组以生药量1 g/mL的加味大柴胡汤10 µL/g灌胃，各组按时间点分别于术后3 d、7 d、10 d取材，每次每组分别随机取10只大鼠。检测各组血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、血清总胆红素（TBIL）水平，实时荧光定量PCR（qPCR）和Western blot分别检测肝组织JNK、Bcl-2的mRNA及蛋白表达情况。结果　O组与M组血清AST、ALT、TBIL均较S组升高，且M组均低于O组（P＜0.05）。O组、M组JNK的mRNA及蛋白水平均高于S组，且M组均低于O组（P＜0.05）；O组、M组Bcl-2的mRNA及蛋白水平均低于S组（P＜0.05），且M组均高于O组（P＜0.05）。结论　加味大柴胡汤能够改善梗阻性黄疸大鼠肝损伤，促进肝组织修复，这可能与下调JNK蛋白表达，促进Bcl-2蛋白表达，从而减弱内质网应激，抑制肝细胞的凋亡有关。

关键词: 大柴胡汤；黄疸, 阻塞性；JNK丝裂原活化蛋白激酶类；基因, bcl-2；肝损伤

Abstract: Abstract: Objective　To investigate the effects of Jiawei Dachaihu decoction on liver injury and expressions of JNK and Bcl-2 in obstructive jaundice rats. Methods　Ninety SD male rats were divided into sham operation group (group S), terrier yellow group (group O) and intervention group (group M) according to random number table method, 30 rats in each group. Group S and group O were gavaged with 10 µL/g of normal saline, group M was gavaged with 10 µL/g of Jiawei Dachaihu decoction with a crude drug amount of 1 g / mL. Each group was sampled at 3 d, 7 d and 10 d after the operation, and 10 rats were randomly taken per group at each point. The serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total biliary red (TBIL) were detected in the three groups. RT-PCR and Western blot assay were used to detect mRNA and protein expressions of liver tissue JNK and Bcl-2, respectively. Results　The serum levels of AST, ALT and TBIL were significantly higher in group O and group M than those in group S, and which were significantly lower in group M than those of group O (P＜0.05). The levels of JNK mRNA and protein were significantly higher in group O and group M than those in group S, and which were significantly lower in group M than those in group O (P＜0.05). The levels of Bcl-2 mRNA and protein were significantly lower in group O and group M than those of group S (P＜0.05), and which were significantly higher in group M than those of group O (P＜0.05). Conclusion　Jiawei Dachaihu decoction can improve liver injury and promote liver tissue repairing in rats with obstructive jaundice, which may be related to the down-regulation of JNK protein, the increase of the expression level of Bcl-2 protein, the weakening of the stress of endoplasmic reticulum and the inhibition of the apoptosis of liver cells. 

Key words: Dachaihu Decoction, jaundice, obstructive, JNK mitogen-activated protein kinases, genes, bcl-2, liver injury

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