天津医药

天津医药 ›› 2021, Vol. 49 ›› Issue (4): 419-423.doi: 10.11958/20203444

• 临床研究 • 上一篇    下一篇

膝骨关节炎患者血清血小板反应蛋白-1水平变化及临床意义#br#

王威威1，尹相云2，邵一鸣2，孙建生2，连鸿凯2△   

  1. 1郑州大学第二附属医院（邮编450014）；2郑州大学附属郑州中心医院
  • 收稿日期:2020-12-11 修回日期:2021-01-01 出版日期:2021-04-15 发布日期:2021-04-16
  • 通讯作者: 连鸿凯 E-mail:lianhongkaidavid@163.com
  • 作者简介:王威威（1995），男，硕士在读，主要从事科疾病的基础和临床研究。E-mail：29971844722@qq.com
  • 基金资助:
    河南省医学科技攻关计划（联合共建）项目（LHGJ20191059）

Changes of serum platelet reactive protein-1 level and its clinical significance in patients with knee osteoarthritis 

WANG Wei-wei, YIN Xiang-yun, SHAO Yi-ming, SUN Jian-sheng, LIAN Hong-kai   

  1. 1 The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China; 
    2 Zhengzhou Central Hospital of Zhengzhou University
  • Received:2020-12-11 Revised:2021-01-01 Published:2021-04-15 Online:2021-04-16
  • Contact: LIAN Hong-kai E-mail:lianhongkaidavid@163.com

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摘要： 目的　探讨膝骨关节炎患者血清中血小板反应蛋白-1（TSP-1）表达的变化及临床意义。方法　选择186例膝骨关节炎患者，依据KL分级标准将患者分为KL 0级组44例、Ⅰ级组52例、Ⅱ级组39例、Ⅲ级组28例、Ⅳ级组23例。另外选取我院健康体检者170例，将其作为对照组。酶联免疫吸附测定（ELISA）试验检测血清中TSP-1水平，分析TSP-1与骨关节炎及临床指标之间的相关性。绘制受试者工作特征（ROC）曲线获得TSP-1预测骨关节炎的最佳临界值。结果　与对照组相比，骨关节炎患者血清TSP-1水平明显升高（P＜0.05）。与KL 0级组的患者相比，KL Ⅰ、Ⅱ、Ⅲ和Ⅳ级组患者血清TSP-1水平显著升高（P＜0.05）。与KL Ⅰ级组患者相比，KⅢ、Ⅳ级组的患者血清TSP-1水平显著升高（P＜0.05）。与KLⅡ、Ⅲ级组的患者相比，KLⅣ级组的患者血清TSP-1水平显著升高（P＜0.05）。血清TSP-1水平与红细胞沉降率（ESR）、血清C反应蛋白（CRP）、软骨寡聚基质蛋白（COMP）、基质金属蛋白酶-13（MMP-13）和转化生长因子-β1（TGF-β1）水平呈正相关。ROC结果示，血清TSP-1诊断骨关节炎的最佳临界值为250.9 μg/L，敏感度为81.1%，特异度为81.9%。结论　骨关节炎患者血清TSP-1水平异常升高，且与骨关节炎的严重程度密切相关。

关键词: 骨关节炎, 膝, 转化生长因子β, KL分级, 血小板反应蛋白-1

Abstract: Objective　To explore the serum expression of thrombospondin-1 (TSP-1) and clinical significance in patients with knee osteoarthritis. Methods　A total of 186 patients with knee osteoarthritis were selected. According to KL classification, the patients were divided into KL 0 group (n= 44), KLⅠgroup (n= 52), KLⅡgroup (n=39), KL Ⅲ group (n= 28) and KL Ⅳ group (n=23). The control group were included 170 healthy people in our hospital. The serum level of TSP-1 was detected by enzyme-linked immunosorbent assay (ELISA), and the correlation between TSP-1 and the severity and clinical indicators of osteoarthritis was analyzed. The subject performance characteristic curve (ROC) was drawn to obtain the optimal threshold value of TSP-1 to predict osteoarthritis. Results　The serum level of TSP-1 was significantly higher in osteoarthritis group than that in control group (P＜0.05). Compared with patients of KL 0 group, the serum TSP-1 levels were significantly higher in KLⅠ, KL Ⅱ, KL Ⅲ and KL Ⅳ groups (P＜0.05). Compared with patients of KLⅠgroup, the serum TSP-1 levels were significantly higher in patients of KL Ⅲ and KL Ⅳ groups (P＜0.05). Compared with patients of KLⅡ and KLⅢgroups, the serum TSP-1 levels were significantly higher in patients of KLⅣgroup (P＜0.05). The serum TSP-1 level was positively correlated with erythrocyte sedimentation rate (ESR), serum C-reactive protein (CRP), cartilage oligomeric matrix protein (COMP), matrix metalloproteinase-13 (MMP-13) and transforming growth factor-β1 (TGF-β1) levels. The ROC results showed that the optimal threshold of serum TSP-1 for the diagnosis of osteoarthritis was 250.9 μg/L (sensitivity=81.1%, specificity=81.9%). Conclusion　The serum level of TSP-1 is abnormally high in patients with osteoarthritis, which is closely related to the severity of osteoarthritis.

Key words: osteoarthritis, knee, transforming growth factor beta, KL classification, thrombospondin-1

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