丹参多酚酸对免疫性不孕大鼠的治疗作用及对下丘脑-垂体-卵巢轴的影响

doi:10.11958/20220990

天津医药 ›› 2023, Vol. 51 ›› Issue (3): 269-273.doi: 10.11958/20220990

丹参多酚酸对免疫性不孕大鼠的治疗作用及对下丘脑-垂体-卵巢轴的影响

柳艾霞¹(), 张庆玲¹, 武志娟^△()

1 济南市第二妇幼保健院中医妇科（邮编250014）
2 山东中医药大学附属医院生殖与遗传科

收稿日期:2022-06-27 修回日期:2022-10-08 出版日期:2023-03-15 发布日期:2023-03-02
通讯作者: 武志娟 E-mail:liuaixia1985l@163.com;luugs84@163.com
作者简介:柳艾霞（1985），女，主治医师，主要从事不孕不育中西医诊治方面研究。E-mail：liuaixia1985l@163.com
基金资助:
山东省中医药科技发展计划项目(2019-0134)

Therapeutic effect of salvia miltiorrhiza polyphenolic acid on immune infertility rats and its effect on hypothalamic-pituitary-ovarian axis

LIU Aixia¹(), ZHANG Qingling¹, WU Zhijuan^△()

1 TCM Gynecology Department of Jinan Second Maternal and Child Health Hospital, Jinan 250014, China
2 Department of Reproduction and Genetics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine

Received:2022-06-27 Revised:2022-10-08 Published:2023-03-15 Online:2023-03-02
Contact: WU Zhijuan E-mail:liuaixia1985l@163.com;luugs84@163.com

柳艾霞, 张庆玲, 武志娟. 丹参多酚酸对免疫性不孕大鼠的治疗作用及对下丘脑-垂体-卵巢轴的影响[J]. 天津医药, 2023, 51(3): 269-273.

LIU Aixia, ZHANG Qingling, WU Zhijuan. Therapeutic effect of salvia miltiorrhiza polyphenolic acid on immune infertility rats and its effect on hypothalamic-pituitary-ovarian axis[J]. Tianjin Medical Journal, 2023, 51(3): 269-273.

摘要/Abstract

摘要： Objective To investigate the therapeutic effect of salvia miltiorrhiza polyphenolic acid on immune infertility rats and its effect on hypothalamus-pituitary-ovarian axis (HPOA). Methods Fifty non-pregnant female SD rats were selected. A rat model of immune infertility with anti-sperm antibody (+) was established by human semen injection immunization. The model rats were divided into the model group (n=9), the prednisone group (n=10), the alvia miltiorrhiza polyphenolic acid low dose group (n=9), the salvia miltiorrhiza polyphenolic acid medium dose group (n=10) and the salvia miltiorrhiza polyphenolic acid high dose group (n=10) by random array table method. Another 10 non-pregnant female rats were selected as the normal control group. The prednisone group was given 5 mg/kg prednisone by intraperitoneal injection, and the salvia miltiorrhiza polyphenolic acid low, medium and high dose groups were given 10, 20 and 40 mg/kg salvia miltiorrhiza polyphenolic acid by intraperitoneal injection. The normal control group and model group were given the same amount of normal saline, once a day for 14 days. The female rats and normal male rats in each group were caged in a ratio of 3∶1 for 2 weeks, and the pregnancy rate of rats in each group was observed. The serum levels of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) were determined by radioimmunoassay. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of rat ovarian tissue. Real-time quantitative reverse transcription polymerase chain reaction (qPCR) was used to detect rat ovarian follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR) and estrogen receptor alpha (ERα) messenger ribonucleic acid (mRNA) expression. Western blotting (WB) was used to detect rat ovarian FSHR, LHR and ERα protein expressions. Results Compared with the normal control group, the conception rate and the serum levels of GnRH, FSH, LH and E2 were decreased in the model group (P<0.05), the corpus luteum and mature follicles in the ovary were significantly reduced, the number of granulosa cell layers was reduced, and there were more cystic follicles, and the mRNA and protein expressions of FSHR, LHR and ERα were decreased (P<0.05). Compared with the model group, the conception rate and the serum levels of GnRH, FSH, LH, and E2 were increased in the prednisone group and the salvia miltiorrhiza polyphenolic acid medium and high dose groups (P<0.05), the number of cystic follicles was significantly decreased, the number of granulosa cell layers was increased, and mature follicles and corpus luteum were formed. The mRNA and protein expressions of FSHR, LHR and ERα were increased (P<0.05). Conclusion Salvia miltiorrhiza polyphenolic acid can promote follicle maturation by regulating HPOA in the treatment of immune infertility.

关键词: 不育，女性, 下丘脑-垂体系统, 促黄体激素, 卵泡刺激素, 受体，LHRH, 丹参多酚酸, 免疫性不孕

Key words: infertility, female, hypothalamo-hypophyseal system, luteinizing hormone, follicle stimulating hormone, receptors, LHRH, salvia miltiorrhiza polyphenolic acid, immune infertility

中图分类号:

R711.6，R593

图/表 5

参考文献 20

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组别	n	GnRH（μg/L）	FSH（IU/L）	LH（IU/L）	E2（ng/L）
正常对照组	10	88.20±15.23	3.73±0.59	6.77±1.24	56.18±10.35
模型组	9	45.72±7.54^a	1.22±0.23^a	3.05±0.56^a	25.24±4.71^a
强的松组	10	70.26±12.71^ab	2.33±0.38^ab	5.14±0.93^ab	42.39±8.04^ab
丹参多酚酸低剂量组	9	42.65±7.37^ac	1.17±0.22^ac	2.78±0.55^ac	24.67±4.51^ac
丹参多酚酸中剂量组	10	54.33±9.15^abcd	1.67±0.28^abcd	4.14±0.78^abcd	34.92±6.13^abcd
丹参多酚酸高剂量组	10	67.83±11.59^abde	2.25±0.37^abde	5.21±0.96^abde	45.80±8.29^abde
F		25.325^＊＊	61.336^＊＊	26.479^＊＊	27.293^＊＊

组别	n	GnRH（μg/L）	FSH（IU/L）	LH（IU/L）	E2（ng/L）
正常对照组	10	88.20±15.23	3.73±0.59	6.77±1.24	56.18±10.35
模型组	9	45.72±7.54^a	1.22±0.23^a	3.05±0.56^a	25.24±4.71^a
强的松组	10	70.26±12.71^ab	2.33±0.38^ab	5.14±0.93^ab	42.39±8.04^ab
丹参多酚酸低剂量组	9	42.65±7.37^ac	1.17±0.22^ac	2.78±0.55^ac	24.67±4.51^ac
丹参多酚酸中剂量组	10	54.33±9.15^abcd	1.67±0.28^abcd	4.14±0.78^abcd	34.92±6.13^abcd
丹参多酚酸高剂量组	10	67.83±11.59^abde	2.25±0.37^abde	5.21±0.96^abde	45.80±8.29^abde
F		25.325^＊＊	61.336^＊＊	26.479^＊＊	27.293^＊＊

组别	n	FSHR	LHR	ERα
正常对照组	10	1.38±0.24	1.77±0.30	2.52±0.45
模型组	9	0.29±0.05^a	0.54±0.09^a	0.49±0.08^a
强的松组	10	1.10±0.21^ab	1.32±0.23^ab	1.79±0.34^ab
丹参多酚酸低剂量组	9	0.33±0.06^ac	0.59±0.11^ac	0.52±0.09^ac
丹参多酚酸中剂量组	10	0.78±0.15^abcd	0.96±0.18^abcd	1.07±0.20^abcd
丹参多酚酸高剂量组	10	1.15±0.22^abde	1.35±0.24^abde	1.84±0.33^abde
F		62.661^＊＊	51.615^＊＊	67.607^＊＊

组别	n	FSHR	LHR	ERα
正常对照组	10	1.38±0.24	1.77±0.30	2.52±0.45
模型组	9	0.29±0.05^a	0.54±0.09^a	0.49±0.08^a
强的松组	10	1.10±0.21^ab	1.32±0.23^ab	1.79±0.34^ab
丹参多酚酸低剂量组	9	0.33±0.06^ac	0.59±0.11^ac	0.52±0.09^ac
丹参多酚酸中剂量组	10	0.78±0.15^abcd	0.96±0.18^abcd	1.07±0.20^abcd
丹参多酚酸高剂量组	10	1.15±0.22^abde	1.35±0.24^abde	1.84±0.33^abde
F		62.661^＊＊	51.615^＊＊	67.607^＊＊

组别	n	FSHR	LHR	ERα
正常对照组	10	0.75±0.13	0.87±0.16	1.12±0.21
模型组	9	0.14±0.02^a	0.26±0.05^a	0.31±0.05^a
强的松组	10	0.49±0.08^ab	0.58±0.10^ab	0.78±0.14^ab
丹参多酚酸低剂量组	9	0.16±0.03^ac	0.24±0.04^ac	0.35±0.06^ac
丹参多酚酸中剂量组	10	0.33±0.06^abcd	0.41±0.07^abcd	0.60±0.11^abcd
丹参多酚酸高剂量组	10	0.52±0.09^abde	0.55±0.09^abde	0.83±0.15^abde
F		71.035^＊＊	55.213^＊＊	48.445^＊＊

丹参多酚酸对免疫性不孕大鼠的治疗作用及对下丘脑-垂体-卵巢轴的影响

Therapeutic effect of salvia miltiorrhiza polyphenolic acid on immune infertility rats and its effect on hypothalamic-pituitary-ovarian axis

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