天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (2): 129-133.doi: 10.11958/j.issn.0253-9896.2015.02.005

• 细胞与分子生物学 • 上一篇    下一篇

线粒体分裂在甲状腺鳞癌SW579 细胞增殖、 凋亡以及侵袭中的作用

申菲菲1 ， 郭睿2 ， 赵树鹏3 ， 齐凤杰1△， 孟翠丽1   

  1. 1辽宁锦州， 辽宁医学院病理学教研室 （邮编121001）； 2西安交通大学第二附属医院病理科； 3辽宁医学院附属第一医院普外科
  • 收稿日期:2014-08-26 修回日期:2014-10-15 出版日期:2015-02-15 发布日期:2015-02-27
  • 通讯作者: 齐凤杰 E-mail:qifj2005@163.com
  • 基金资助:
    辽宁省科学技术计划项目 （2010225034）

Effects of mitochondrial fission in the cell proliferation ,apoptosis and invasion of thyroid squamous cell carcinoma cell line SW579

  • Received:2014-08-26 Revised:2014-10-15 Published:2015-02-15 Online:2015-02-27

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摘要： 摘要： 目的 研究线粒体动力学蛋白 Mfn2 和 Drp1 在甲状腺鳞癌 SW579 细胞中的表达和线粒体分裂抑制剂-1 （Mdivi-1） 对 SW579 细胞增殖、 凋亡及侵袭能力的影响。方法 采用 RT-PCR 检测 Mfn2 和 Drp1 mRNA， Western blot 检测 Mfn2 和 Drp1 蛋白在 Nthy-ori 3-1 和 SW579 两种细胞中的表达； 然后将 SW579 分为对照组 （DMSO, 0.1%）和 Mdivi-1 低、 中、 高剂量组 （浓度分别为 15、 30、 45 μmol/L）， 培养 16 h， 采用 MTT 法检测细胞增殖能力的变化， 用荧光分光光度计检测线粒体膜电位的变化， 用 RT-PCR 检测细胞色素 C （Cyt C） 和 Caspase-3 mRNA 的变化， Western blot 检测 Cyt C 和 Caspase-3 蛋白的变化， 用 Transwell 小室侵袭实验检测侵袭能力的变化。结果 与 Nthy-ori 3-1 细胞系相比， SW579 细胞系中 Mfn2 mRNA 和蛋白表达明显降低， Drp1 mRNA 和蛋白表达明显升高 （P＜0.01）； 与对照组相比， Mdivi-1 各组 SW579 细胞存活率和线粒体膜电位均明显降低， Cyt C 和 Caspase-3 的 mRNA 及蛋白表达均明显增加， 侵袭能力明显减弱 （均 P＜0.01）， 并且呈药物浓度依赖性。结论 甲状腺鳞癌 SW579 细胞中存在线粒体动力学异常， Mdivi-1可以抑制此细胞的增殖和侵袭， 并能诱导其凋亡。

关键词: 甲状腺肿瘤, 癌, 鳞状细胞, 细胞增殖, 细胞凋亡, 线粒体动力学, Mdivi-1, Mfn2, Drp1

Abstract: ObjectiveTo detect the changes of mitochondrialdynamicsproteins(Mfn2 and Drp1) in cell line SW579, and the effects of Mdivi-1 on cell proliferation,apoptosis and invasion of cell line SW579. Methods In SW579 and Nthy-ori 3-1, the expression levels of Mfn2 and Drp1 were determined by western blot, the Mfn2 and Drp1 mRNA expression levels were measured by RT- PCR .Then, SW579 cells were divided into control group(DMSO, 0.1%)and Mdivi-1 group (different concentrations of Mdivi-115，30 and 45 μmol/L). SW579 cells were treated with Mdivi-1 for 16 h。Then the ability of cell proliferation was detected using MTT assay,the mitochondrial membrane potential was detected by fluorescence spectrophotometer, the expression levels of cytochrome C and Caspase-3 were measured by Western blot, the Cyt C and Caspase-3 mRNA expression levels were determined by RT- PCR. the ability of invasion in each group was measured with Transwell assays. Results Compared with Nthy-ori 3-1,The mRNA and protein expression of the Mfn2 was remarkably decreased, whilethe mRNA and protein expression of the Drp1 was significantly increased in SW579 (P＜0.01). Compared with the control group, the cell survival rates and the mitochondrial membrane potential of SW579 weredecreased dramatically(P＜0.01),the mRNA and protein expression of the cytochrome C and Caspase-3 were increased dramatically (P＜0.01), the capability of invasion was markedly decreased in the Mdivi-1 group (P＜0.01) , which are all in a concentration-dependent manners (P＜0.01). Conclusion Abnormal mitochondrial dynamics may be involved in thyroid squamous cell carcinomaSW579 cells; Mdivi-1 can inhibit the cell proliferation as well as invasion, and also induce apoptosis.

Key words: thyroid neoplasms, carcinoma, squamous cell, cell proliferation, apoptosis, mitochondrial dynamics, Mdivi- 1, Mfn2, Drp1