天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (2): 162-165.doi: 10.11958/j.issn.0253-9896.2015.02.013

• 实验研究 • 上一篇    下一篇

吗啡急性处理小鼠的微阵列数据分析和生物标记识别

张文华， 王燕平△   

  1. 1黑龙江齐齐哈尔， 齐齐哈尔医学院附属第三医院麻醉科 （邮编161000）； 2济南， 济南循证医药科技开发中心科研
  • 收稿日期:2014-07-29 修回日期:2014-08-28 出版日期:2015-02-15 发布日期:2015-02-27
  • 通讯作者: 王燕平 E-mail:513910531@qq.com
  • 基金资助:
    2012年国家医学教育发展中心课题项目 （2012-03-07-142）

Microarray datasets and biomarker in mice who were acutely administered with morphine

  • Received:2014-07-29 Revised:2014-08-28 Published:2015-02-15 Online:2015-02-27

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摘要： 摘要： 目的 通过生物信息学方法， 识别吗啡急性处理小鼠的差异表达基因及其富集的通路。方法 从高通量基因表达数据库(Gene Expression Omnibus)下载吗啡急性处理小鼠的微阵列数据， 并调用 R 语言 3.1.0 软件的质量控制包 AffyQCReport 1.42.0 对数据样本进行质量控制分析， 运用 R 语言的微阵列数据线性模型识别吗啡处理前后的差异表达基因， 采用表达分析系统检测算法进行差异表达基因的通路富集分析。结果 吗啡急性处理小鼠的微阵列基因表达数据具有良好的均一性和阵列强度相似性， 识别得到 Gm11627、 Zfand4、 Zbtb16、 Pkp2 和 Plin4 等 481 个差异表达基因和癌症、 黑素原生成和丝裂原活化蛋白激酶信号等 8 条代谢通路。结论 所识别的差异表达基因和代谢通路成为吗啡急性处理小鼠潜在的生物标记。

关键词: 吗啡, 小鼠, 微阵列分析, 基因表达, 生物学标记, 功能富集分析

Abstract: Abstract： Objective To identify the differentially expressed genes and functional enrichment pathways using bioin⁃ formatics technology in mice who were acutely administered with Morphine. Methods First, we downloaded microarray da⁃ tasets of mice which were acutely administered with Morphine from Gene Expression Omnibus, and assess the quality control parameters of the microarray datasets using AffyQCReport 1.42.0 package by R programming language 3.1.0 software. Subse⁃ quently, the differentially expressed genes of the microarray datasets were identified using the linear models for microarray data of R language. Finally, functional pathways of the differentially expressed genes were enriched based on expression anal⁃ ysis and systematic explorer. Results The microarray datasets showed preferable uniformity and intension similarity. A to⁃ tal of 481 differentially expressed genes including Gm11627, Zfand4, Zbtb16, Pkp2 and Plin4 were identified. While 8 func⁃ tional enrichment pathways, including pathways in cancer, melanogenesis and mitogen-activated protein kinase signaling were revealed. Conclusion The differentially expressed genes and functional pathways were the underlying biomarkers of mice who were acutely administered with Morphine.

Key words: morphine, mice, microarray analysis, gene expression, biological markers； enrichment of functional analysis