天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (6): 674-676.doi: 10.11958/j.issn.0253-9896.2015.06.026

• 诊断技术 • 上一篇    下一篇

miR-125b、miR-29a和miR-155-5p作为诊断结核性脑膜炎生物标志的研究

柴璐璐 1， 田宋新 1， 袁俐 2， 王宏 1   

  1. 1新疆石河子大学医学院第一附属医院神经内科 （邮编832000）； 2新疆石河子大学医学院病原微生物与免疫学教研室
  • 收稿日期:2014-12-01 修回日期:2015-01-22 出版日期:2015-06-15 发布日期:2015-06-10
  • 通讯作者: 柴璐璐 E-mail:qian66qian@yeah.net
  • 基金资助:
    国家 “十二五” 计划重大传染病防治专项结核病专项 （2013ZX10003003-002-002）

Study of miR-125b, miR-29a and miR-155-5p as biomarkers of tuberculous meningitis

CHAI Lulu1,TIAN Songxin1,YUAN Li2 ,WANG Hong1   

  1. 1 Department of Neurology , the First Affiliated Hospital,Medicine School of Shihezi University, Shihezi 832000,China;
    2 Department of Pathogenic Microbiology and Immunology,Medicine School of Shihezi University

  • Received:2014-12-01 Revised:2015-01-22 Published:2015-06-15 Online:2015-06-10

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摘要： 目的 探讨结核性脑膜炎患者脑脊液及血浆中 microRNA (miR)-125b、 miR-29a 和 miR-155-5p 的表达水平及其临床意义。方法 收集 20 例结核性脑膜炎患者（结脑组）和 20 例原发性头痛患者（对照组）的脑脊液和血浆， 分别提取总 RNA， 使用实时定量 PCR 方法分别检测脑脊液和血浆中 miR-125b、 miR-29a 及 miR-155-5p 的表达水平。结果 与对照组相比， 结脑组脑脊液、 血浆中 miR-29a、 miR-125b 表达水平明显上调， 结脑组血浆中 miR-155-5p 表达水平明显上调， 差异均有统计学意义 （均 P<0.01）， 而 2 组脑脊液中 miR-155-5p 表达水平比较差异无统计学意义。结论 miR-125b、 miR-29a 和 miR-155-5p 可能参与了调节结核性脑膜炎的发生、 发展过程， miR-125b和 miR-29a 有可能作为潜在的诊断结核性脑膜炎的生物学标志。

关键词: 结核, 脑膜, 血浆, 脑脊髓液, 微 RNAs, 实时定量 PCR, miR-125b, miR-29a, miR-155-5p

Abstract: Objective To investigate the expression levels of microRNA(miR)-125b, miR-29a and miR-155-5p in cerebrospinal fluid (CSF) and plasma from the patients with tuberculous meningitis and their clinic significance. Methods Cerebrospinal fluid and plasma samples were collected from 20 patients with tuberculous meningitis (tuberculous meningitis group) and 20 patients suffered from primary headache (control group). The total RNAs were extracted.The levels of miR-
125b, miR-29a and miR-155-5p were determined by real-time quantitative PCR (q-PCR). Results Levels of miR-29a and miR-125b in both CSF and plasma of patients in tuberculous meningitis group were significantly higher than those in patients from control group with statistical significance (P<0.01). Mean time, the level of miR-155-5p in plasma but not in CSF of patients in tuberculous meningitis group was higher than those in control group with statistical significance (P<0.01). Conclusion Expression of miR-125b, miR-29a and miR-155-5p may participate in regulating the occurrence and development of tuberculous meningitis. And miR-125b and miR-29a may be used as potential biomarkers for diagnosing tuberculous meningitis.

Key words: tuberculosis, meningeal, plasma, cerebrospinal fluid, microRNAs, real-time quantitative PCR, miR-125b,
miR-29a, miR-155-5p