天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (10): 1116-1118.doi: 10.11958/j.issn.0253-9896.2015.10.008

• 实验研究 • 上一篇    下一篇

新生小鼠缺氧缺血性脑病脑组织 caspase-3#br# 表达的变化

  

  1. 1江西中医药大学生理学学科组 （邮编330004）， 2针灸康复科， 3解剖学学科组， 4实验动物科技中心
  • 收稿日期:2015-04-03 修回日期:2015-06-20 出版日期:2015-10-15 发布日期:2015-10-22
  • 基金资助:
    江西中医药大学课题;江西省卫生计生委中医药科研计划项目

Increased expression of caspase-3 in ipsilateral neonatal hypoxic-ischemia encephalopathy#br# model in mice

  1. 1 Department of Physiology, 2 Department of Rehabilitation of Affiliated Hospital, 3 Department of Anatomy, 4 Animal Experi?
    ment Center of Science and Technology, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
  • Received:2015-04-03 Revised:2015-06-20 Published:2015-10-15 Online:2015-10-22

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摘要：

摘要： 目的 观察半胱氨酸天冬氨酸蛋白酶 （caspase） -3 在新生儿缺氧缺血性脑病 （NHIE） 小鼠模型脑组织中表
达的变化。方法 选取 7 d CD1 新生小鼠 30 只， 按随机数字表法分为假手术组 （9 只） 和 NHIE 模型组 （21 只）， 后者
制备 NHIE 动物模型。用 TTC 染色法检查 2 组的脑组织梗死面积； DAPI 染色观察脑组织病理变化； 原位末端标记
技术检测脑组织细胞凋亡； 荧光免疫组化法检测脑组织中 caspase-3 表达水平。结果 假手术组小鼠脑组织未见梗
死灶， 脑组织细胞排列致密整齐， TUNEL 阳性细胞数（ [ 18.57±4.98） 个]和 caspase-3 阳性细胞数[(9.17±2.14)个]明显低
于 NHIE 模型组的 TUNEL 阳性细胞数（ [ 209.57±41.27） 个]和 caspase-3 阳性细胞数（ [ 63.33±16.22） 个]； 与假手术组相
比， NHIE 模型组小鼠的右侧半球可见梗死灶， 脑组织细胞大量坏死脱落、 周围组织间隙变大。结论 NHIE 模型鼠
出现的脑组织损伤可能与 caspase-3 表达增加、 脑组织细胞凋亡增加有关。

关键词: 缺氧缺血, 脑梗死, 细胞凋亡, 半胱氨酸天冬氨酸蛋白酶 3, 小鼠, 缺氧缺血性脑病

Abstract:

Abstract：Objective To observe apoptotic cells and caspase-3-positive cells in ipsilateral neonatal hypoxic-isch⁃
emia encephalopathy (NHIE) model in mice. Methods CD1 mice of age 7 days (n=30) were randomly divided into two
groups: sham group (n=9) and model group (n=21). NHIE model was induced by right common carotid artery ligation fol⁃
lowed by 8% oxygen hypoxia for 100 min. TTC staining was used to determine area of brain infarction. DAPI staining was
used to detect pathological change in brains. TUNEL assay was used to detect apoptotic cells and fluorescence immunohisto⁃
chemistry was used to detect caspase-3 expression in the ipsilateral brain. Results No infarct was detected in sham group.
Cells were densely and orderly arranged in brain. TUNEL-positive cells (18.57±4.98) and caspase-3-positive cells (9.17±
2.14) in the ipsilateral brain were both less than those in the ipsilateral brain of mice in model group (209.57±41.27) and
(63.33±16.22) respectively. Mice in model group presented infarct in the right hemisphere with more dead cells and wider in⁃
terstitial space compared with sham group. Conclusion Brain injury in NHIE model might be related to the increasing cas⁃
pase-3 expression thus leads to apoptosis.

Key words: hypoxia-ischemia, cerebral infarction, apoptosis, caspase 3, mice, hypoxic-ischemic encephalopathy