天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (10): 1144-1146.doi: 10.11958/j.issn.0253-9896.2015.10.016

• 实验研究 • 上一篇    下一篇

荷丹片对APOE-/-小鼠炎症因子及氧化应激因子的影响

  

  1. 1河北保定， 河北大学研究生院 （邮编071000）； 2基础医学院药理教研室； 3河北大学附属医院； 4沧州市人民医院
  • 收稿日期:2015-01-30 修回日期:2015-05-05 出版日期:2015-10-15 发布日期:2015-10-22

Effects of Hedan tablet on cytokins and oxidative stress factors in APOE-/- mouse

  1. 1 Graduate College, Hebei University, Baoding 071000, China; 2 Basic Medical College, Hebei University;
    3 Affiliated Hospital of Hebei University; 4 the People′s Hospital of Cangzhou
  • Received:2015-01-30 Revised:2015-05-05 Published:2015-10-15 Online:2015-10-22

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摘要：

摘要： 目的 探讨荷丹片对载脂蛋白 E 基因敲除 （APOE-/-） 小鼠炎症因子、 氧化应激因子的影响及对动脉粥样硬
化（AS）的干预作用及机制。方法 将 50 只 APOE-/-小鼠随机均分为对照组、 模型组、 荷丹片低剂量组、 荷丹片高剂
量组及辛伐他汀组。对照组给予普通饲料， 其余组给予高胆固醇饲料造模。同时用药组灌胃相应剂量药物， 对照组
和模型组给予等量生理盐水灌胃。造模 12 周后测定血清白细胞介素（IL） -1、 IL-10、 丙二醛（MDA）、 超氧化物歧化
酶（SOD）水平； RT-PCR 测主动脉肿瘤坏死因子（TNF） -α mRNA 表达。结果 模型组较对照组 IL-1、 MDA、 TNF-α
mRNA 升高， IL-10、 SOD 降低 （P < 0.01）； 荷丹片低剂量组、 荷丹片高剂量组、 辛伐他汀组较模型组 IL-1、 MDA、 TNF-
α mRNA 降低， IL-10、 SOD 升高 （P < 0.01）。结论 荷丹片可通过抗炎、 抗氧化应激作用干预 AS 的发生、 发展。

关键词: 动脉粥样硬化, 载脂蛋白 E 类 , 模型, 动物 , 荷丹片, APOE-/-小鼠, 炎症因子, 氧化应激因子

Abstract:

Abstract: Objective To observe the effects of Hedan tablet on cytokines and oxidation factors in APOE-/- mouse, and
to explore its effect on atherosclerosis and to explore its behind mechanism. Methods APOE-/- mice (n=50) were randomly
divided into control group, model group, low dose Hedan tablet treatment group, high dose Hedan tablet treatment group and
simvastatin treatment group. Mice in control group were given normal feed while mice in other groups were fed with high cho⁃
lesterol diet. Hedan or Simvastatin was administrated intra-gastrically while normal saline was given to model group in the
same route. After 12 weeks, mice were sacrificed to observe the mRNA level of tumor necrosis factor-α(TNF-α mRNA) in
aorta by RT-PCR. Mean while, serum levels of interleukin-1 (IL-1), interleukin-10 (IL-10), malonaldehyde (MDA) and su⁃
peroxide dismutase (SOD) were determined in different groups. Results Compared with control group, TNF-α mRNA tran⁃
scription level as well as serum levels of IL-1 and MDA significantly increase while serum levels of IL- 10 and SOD de⁃
creased remarkably in model group, (P < 0.01). Compared with model group, mRNA levels of TNF-α as well as serum levels
of IL-1 and MDA were significantly decreased while serum levels of IL-10, SOD were greatly increased in low dose and high
dose Hedan tablet treatment groups as well as in simvastatin treatment group (P < 0.01). Conclusion Hedan tablet inhibit
the formation of atherosclerosis through its anti-oxidation role and anti-inflammation role.

Key words: atherosclerosis, apolipoproteins E, models, animal, Hedan tablet, atherosclerosis, APOE-/- mouse, inflam?
mation factor, oxidation stress factor