天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (12): 1353-1355.doi: 10.11958/j.issn.0253-9896.2015.12.003

• 细胞与分子生物学 • 上一篇    下一篇

miR-320-3p 调控脂肪细胞分化的研究

王昌兰1,2，高志红2，常爱玲1,2，王宝利1△   

  1. 1天津医科大学代谢病医院内分泌研究所，卫生部激素与发育重点实验室（邮编300070）；2天津医科大学总医院内分泌科
  • 收稿日期:2015-05-22 修回日期:2015-08-02 出版日期:2015-12-15 发布日期:2015-12-11
  • 通讯作者: △通讯作者E-mail：bliwang72@163.com E-mail:865777458@qq.com
  • 作者简介:王昌兰（1987），女，硕士，主要从事基因分子生物学研究
  • 基金资助:
    国家自然科学基金资助项目（81271977，81472040）

The impact of miR-320-3p on adipocyte differentiation

WANG Changlan1,2, GAO Zhihong2, CHANG Ailing1,2, WANG Baoli1△   

  1. 1 Key Laboratory of Hormones and Development (Ministry of Health), Metabolic Diseases Hospital & Institute of Endocrinology, Tianjin Medical University, Tianjin 300070,China; 2 Department of Endocrinology, General Hospital of Tianjin Medical University
  • Received:2015-05-22 Revised:2015-08-02 Published:2015-12-15 Online:2015-12-11
  • Contact: △Corresponding Author E-mail：bliwang72@163.com E-mail:865777458@qq.com

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摘要： 目的探讨miR-320-3p 对脂肪细胞分化的影响。方法分离并培养小鼠骨髓间充质干细胞（MSCs），并对其进行脂肪细胞诱导分化3 d，用qRT-PCR 检测miR-320-3p 在成脂分化过程中的表达水平。在骨髓基质细胞系 ST2 中转染miR-320-3p，对其进行成脂方向诱导分化，用油红O 染色和qRT-PCR 检测miR-320-3p 对ST2 成脂分化的影响。结果MSCs 向脂肪细胞分化过程中miR-320-3p 表达增加（P < 0.01）；与转染阴性对照NC mimics 相比，ST2 细胞转染miR-320-3p mimics 后油红O 染色显示脂肪细胞明显增多，脂肪细胞特异性转录因子过氧化物酶体增殖物激活受体γ(PPARγ)、CCAAT 增强子结合蛋白α（C/EBPα）和脂肪细胞脂肪酸结合蛋白4（FABP4）基因表达显著升高，差异有统计学意义（P < 0.05）。结论miR-320-3p 可促进ST2 向脂肪细胞分化。

关键词: 脂细胞, 间质干细胞, 细胞分化, 成脂分化, 微RNAs, miR-320-3p

Abstract: Objective To study the role of miR-320-3p in adipocyte differentiation. Methods Marrow mesenchymal stem cells were isolated from mice and cultured then induced with adipogenic agents for 3 days. The transcription level of miR-320-3p was examined by qRT-PCR. Stromal ST2 cells were transfected with miR-320-3p, followed by adipogenic treatment. Oil-red O staining and qRT-PCR were employed to assess the differentiation of adipocytes induced by miR-320- 3p. Results The expression level of miR-320-3p increased in MSCs after adipogenic treatment (P < 0.01). Addition of miR-320-3p in ST2 cells promoted the formation of oil-red O positive adipocytes and up-regulated the expression levels of adipogenic transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein-α (C/EBPα) and the marker gene adipocyte fatty acid binding protein 4 (FABP4)，compared to cells that transfected with miR-320-3p mimics (P < 0.05). Conclusion miR-320-3p promotes ST2 cells differentiation into adipocytes.

Key words: adipocytes, mesenchymal stem cells, cell differentiation, adipogenesis, microRNAs, miR-320-3p