天津医药

天津医药 ›› 2014, Vol. 42 ›› Issue (12): 1176-1179.doi: 10.3969/j.issn.0253-9896.2014.12.007

• 实验研究 • 上一篇    下一篇

β2-GP1、VEGF、TF与大鼠DVT形成的关系

宋恩1,李光第2,娄振凯2,赵学凌1   

  1. 1. 昆明医科大学第一附属医院骨科
    2. 昆明医科大学第一附属医院
  • 收稿日期:2014-08-22 修回日期:2014-08-30 出版日期:2014-12-15 发布日期:2014-12-15
  • 通讯作者: 赵学凌 E-mail:zhaoxuelin@vip.km169.net
  • 基金资助:
    国家自然科学基金资助项目;云南省卫生科技计划项目;云南省科技厅重点新产品开发计划项目

The Correlation between β2-GP1 ， VEGF and TF with Rat DVT Formation

SONG En,Guang-Di LI,LOU Zhenkai ,ZHAO Xueling   

  1. Orthopedics Department of the First Affiliated Hospital of Kunming Medical University
  • Received:2014-08-22 Revised:2014-08-30 Published:2014-12-15 Online:2014-12-15
  • Contact: ZHAO Xueling E-mail:zhaoxuelin@vip.km169.net

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摘要：

【摘要】 目的 建立大鼠下腔静脉狭窄法深静脉血栓（DVT）模型， 检测大鼠血液中β-2 糖蛋白 1（β2-GP1）、血管内皮生长因子（VEGF）、组织因子（TF）表达变化， 研究其与 DVT 形成的关系。 方法 70 只 SD 大鼠， 随机分为对照组（10 只）， 假手术组（30 只）， 模型组（30 只）， 模型组于造模后 2 h、8 h、24 h 分为 3 个亚组， 采集各组大鼠下腔静脉血 5 mL， ELISA 法检测β2-GP1、VEGF、TF 水平。 结果 大鼠血液中β2-GP1、VEGF、TF 表达在对照组与假手术组间比较无统计学差异（P＞ 0.05）， 造模后 2～ 24 h， β2-GP1、VEGF、TF 在血液中的表达呈上升趋势， 24 h 时达峰值， 高于对照组、假手术组、造模后 2 h 组和造模后 8 h 组（P＜ 0.01）， 其余 4 组间比较差异无统计学意义（P＞ 0.05）。 结论大鼠 DVT 形成过程中， β2-GP1、VEGF、TF 可能起到促进血栓形成的作用。

关键词: β2糖蛋白1, 血管内皮生长因子, 深静脉血栓, 凝血致活酶, 静脉血栓形成, 大鼠, Sprague-Dawley

Abstract:

[Abstract] Objective To build rat DVT inferior vena cava partial stasis (narrow) model, to detected the expression of β2-GP1, VEGF and TF in rat blood, and to investigat the correlation between β2-GP1, VEGF and TF with DVT. Methods SD rats (n=70) are divided into control group (n = 10), sham operation group (n = 30) and the model group (n = 30) ran? domly and DVT model was built by the inferior vena cava partial stasis (narrow) after 2 h, 8 h and 24 h respectively. In each time point, ten rats were taken in each group, inferior vena cava blood were collected while β2-GP1, VEGF and TF expres? sion were detected by ELISA. Results In rat experiment, compared with control group, there was no significant change in? expression of β2-GP1, VEGF and TF in sham operation group (P ＞ 0.05). Levels of β2-GP1, VEGF and TF were in? creased at the 2nd hour and 8th hour then peak at the 24th hour which was higher than those in the 24th hour control group and in Sham group and it was also higher than those in the 2nd hour and the 8th hour in model group with statistical signifi? cant difference (P ＜ 0.01). Conclusion Based on the above experimental data, in rat DVT formation process, β2-GP1, VEGF and TF may play an important role in promote DVT formation.

Key words: β-2 glycoprotein 1, VEGF, Deep venous thrombosis, thromboplastin, venous thrombosis, rats, Sprague-Dawley