天津医药

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G蛋白在快速老化痴呆小鼠SAMP8脑组织中的异常表达

阚伯红1,张海霞2,韩景献1,于建春1   

  1. 1. 天津中医药大学第一附属医院
    2. 天津中医药大学第一附属医药
  • 收稿日期:2010-04-27 修回日期:2010-06-10 出版日期:2010-11-15 发布日期:2010-11-15
  • 通讯作者: 阚伯红

Abnormal expression of G protein in the brain of the senescence-accelerated prone mouse(SAMP8)

  • Received:2010-04-27 Revised:2010-06-10 Published:2010-11-15 Online:2010-11-15
  • Contact: Kan Bo hong

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摘要： 摘要 目的：探索在不同月龄小鼠SAMP8皮层和海马中G蛋白的异常表达情况。方法：以正常老化小鼠SAMR1为对照，利用Western-blot方法检测Gαq/11、Gαi和GαS蛋白在8月龄和18月龄小鼠SAMP8的异常表达。结果：与同月龄SAMR1相比，GαS、Gαi和Gαq蛋白在8月龄小鼠SAMP8皮层和海马组织中的表达无明显异常（p﹥0.05），而到18月龄时，SAMP8皮层部位Gαq表达量明显低于SAMR1（p﹤0.05）,海马部位Gαq和 Gαi的表达量也明显低于SAMR1（p﹤0.05）。结论：18月龄SAMP8脑组织中Gαq和 Gαi的异常表达参与了阿尔海默病（Alzheimer’s, AD）的病理进展，并有望作为AD治疗的一个靶点。

关键词: G蛋白, SAMP8, 阿尔海默病, Western-blot

Abstract: Abstract Objective: To explore abnormal expression of G protein in the cortex and hippocampus of 8-old-month and 18-old-month senescence-accelerated prone mouse(SAMP8). Methods: senescence-accelerated mice resistant 1(SAMR1) as control mice, Detection of Gαq/11, Gαi and GαS protein abnormal expression in 8-month-old and 18-month-old SAMP8 by using Western-blot method. Results: Compared with the month-old SAMR1, expression of GαS, Gαi and Gαq protein in 8-month-old SAMP8 cortex and hippocampus were not different (p> 0.05). Gαq expression in 18-months-old SAMP8 cortex was significantly lower than that of SAMR1 (p <0.05). Gαq and Gαi expression in 18-months-old SAMP8 hippocampus was significantly lower than SAMR1(p <0.05). Conclusion: Abnormal expression of Gαq and Gαi in 18-month-old SAMP8 brain involved in Alzheimer Disease (Alzheimer's, AD) pathological progress and is expected to be a target for AD treatment.

Key words: G protein, SAMP8, Alzheimer disease, Western-blot