天津医药

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低氧刺激GES-1细胞后 MICA基因在mRNA和蛋白水平的表达

张晓岩1,张先钧2,王海燕2,蒲小燕2,贾炜2   

  1. 1. 青海大学医学院组胚教研室
    2.
  • 收稿日期:2009-11-11 修回日期:2010-01-30 出版日期:2010-07-15 发布日期:2010-07-15
  • 通讯作者: 张晓岩

Expression of MICA at the mRNA and protein levels in Human Gastric Epithelial cell Strain-1 of Hypoxia

  • Received:2009-11-11 Revised:2010-01-30 Published:2010-07-15 Online:2010-07-15

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摘要： 【摘　要】目的:研究低氧对体外培养胃上皮细胞株GES-1中MICA 基因表达的影响。方法:建立GES-1细胞体外低氧培养模型。在缺氧处理后(12h、24h、72h) ,RT -PCR 和免疫细胞化学技术分别检测MICA 基因和蛋白的表达变化。结果:随缺氧增加,免疫细胞化学及RT-PCR证实MICA 蛋白的表达与MICAmRNA 的表达同步, 且随着缺氧时间的增加呈时间效应关系。且表达量随缺氧时间而升高,Pearson级差相关分析显示MICA mRNA和蛋白水平表达显著正相关。结论:低氧氧可以显著增加胃上皮细胞株GES-1中MICA 基因的表达。

关键词: 低氧, MHC-I类链相关蛋白A, 人胃粘膜上皮细胞

Abstract: 【Abstract】Objective :To investigate the effect of hypoxia on MICA expression in GES-1 its mechanism. Methods :Hypoxia culture model in vitro was established to test the expression of MICA in GES-1 cell line. After cultured in hypoxia circumstance for a given period of time (12hours ,24hours ,72hours ) ,MICA expression was tested in mRNA level and protein level by RT - PCR and immunocytochemistry analysis . Results: Cell survival was affected a little within 20 hours under hypoxia. MICA expressions increased after treatment of hypoxia. MICA protein expressions increased equally with mRNA confirmed by immunocytochemistry and RT - PCR and its positive correlation was found by Pearson product - moment correlation analysis. Conclusion :MICA gene and protein can be induced remarkably byhypoxia in GES-1.

Key words: Hypoxia, MICA, GES-1