康脑液对脑缺血再灌注损伤大鼠血清及脑组织中氨基酸含量的影响

康脑液对脑缺血再灌注损伤大鼠血清及脑组织中氨基酸含量的影响

石会娟

河北北方学院

收稿日期:2011-02-21 修回日期:2011-05-12 出版日期:2011-10-15 发布日期:2011-10-15
通讯作者: 石会娟

Effect of KangNaoYe on the AA contents in serum and brain tissue in rats during Cerebral ischemia-reperfusion

Received:2011-02-21 Revised:2011-05-12 Published:2011-10-15 Online:2011-10-15

石会娟. 康脑液对脑缺血再灌注损伤大鼠血清及脑组织中氨基酸含量的影响[J]. .

摘要/Abstract

摘要： [摘要]目的:观察康脑液对脑缺血再灌注损伤大鼠血清及脑组织中氨基酸含量的影响，并探讨其作用机制。方法：将大鼠随机分6组，假手术组、模型组、康脑液低、中、高剂量组、依达拉奉组。采用线栓法制作模型，相应时间点取脑，应用高效液相色谱法测定血清及脑组织中Asp、Glu、Gly、γ-GABA含量。结果：康脑液能够降低血清及脑组织Asp、Glu含量，升高Gly、γ-GABA含量，与模型组比较差异显著（P<0.05~0. 01）。结论：康脑液能够减轻大鼠脑缺血再灌注损伤，机制可能与其干扰血液及脑组织内氨基酸代谢有关。

关键词: 康脑液, 脑缺血再灌注损伤, 高效液相色谱法, 氨基酸

Abstract: Abstract: Objective:To observe the effect of KangNaoYe on the AA contents in serum and brain tissue in rats during cerebral ischemia-reperfusion in order to investigate the mechanism of action of KangNaoYe.Methods: Rats were randomly divided into 6 groups,such us sham-operation grop, ischemia-reperfusion grop, KangNaoYe grops with different dose, Edaravone grop. The models of cerebral ischemia-reperfusion were established in rats by methods of MCAO（middle cerebral artery occlusion）.The animals were killed to the deadline of the schedule.The contents changes of Asp,Glu,Gly,γ-GABA in serum and brain tissue were were detected by reversed-phase High Performance Liquid Chromatography with pre-column derivatization respectively. Results: Compared with ischemia-reperfusion grop, Gly,γ-GABA activities were increased significantly , Asp,Glu content were decreased significantly in KangNaoYe groups(P<0.05~0.01). Conclusion: KangNaoYe shows protective effects on cerebral ischemia-reperfusion. The mechanism is that KangNaoYe can mediate the level of AA in blood serum and brain tissue.

Key words: KangNaoYe, cerebral ischemia-reperfusion, HPLC, amine acid

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