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颅内动脉瘤性蛛网膜下腔出血患者血清血管生成素-1/-2的表达

王毅1,吴凤琪2,张建宁1,向毅3   

  1. 1. 天津医科大学总医院
    2. 天津市第一中心医院
    3. 天津市环湖医院
  • 收稿日期:2011-02-21 修回日期:2011-05-28 出版日期:2011-11-15 发布日期:2011-11-15
  • 通讯作者: 王毅

Abnormal Serum Expression of the Angiopoietin-1/-2 in Aneurysmal Subarachnoid Hemorrhage

  • Received:2011-02-21 Revised:2011-05-28 Published:2011-11-15 Online:2011-11-15
  • Contact: Yi WANG

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摘要： 摘要 目的：探讨颅内动脉瘤性蛛网膜下腔出血（aSAH）患者血清血管生成素（Ang）表达水平变化的临床意义。 方法：记录正常查体者40例（对照组）和经影像学诊断证实的aSAH患者31例（研究组）的一般资料及aSAH患者Hunt-Hess临床分级；采集其外周血3ml，使用促凝剂/分离胶真空抽血管，4℃离心15min，取血清-80℃保存，采用ELISA法检测血清Ang-1与Ang-2表达水平。使用统计学方法进行数据分析。 结果：与正常查体者组（对照组）比较，aSAH组（研究组）SBP、DBP、血清Ang-1和Ang-2表达水平均升高，差异有统计学意义（t=-8.844, P=0.000；t=-5.316, P=0.000；t=-8.858, P=0.000；t=-2.371, P=0.022）。依照ICA的Hunt-Hess临床分级，aSAH重症患者（IV～V）血清Ang-1 、Ang-2和SBP表达水平高于轻症患者（I～III），差异有统计学意义（Z=-3.928, P=0.000; Z=-3.657, P=0.000；Z=-2.127, P=0.034）。 结论：研究结果提示，Ang-1和Ang-2的异常表达可能在ICA从发生到破裂的病理生理过程中发挥了重要的作用，Ang-1/ Ang-2比例有可能是ICA破裂和aSAH严重程度的重要指标之一。

关键词: 血管生成素, 颅内动脉瘤, 蛛网膜下腔出血, Hunt-Hess分级, 高血压, 血清, 酶联免疫法

Abstract: Abstract Objective:To investigate the effect of the levels of serum Ang-1 and Ang-2 in aSAH. Methods: We carried out a free aSAH screening in Tianjin. Serum samples were collected as follows: 40 from normal subjects, 31 from patients with aSAH . All the patients were diagnosed by CT and 3D-DSA. Serum levels of Ang1, Ang2 and Tie-2 were tested and compared by enzymelinked immunosorbent assay (ELISA). Results: The levels of serum Ang-1 (t=-8.858, P=0.000), Ang-2 (t=-2.371, P=0.022), SBP (t=-8.844, P=0.000) and DBP (t=-5.316, P=0.000) of aSAH patients had significant higher levels , when compared with healthy subjects. The levels of serum Ang-1,Ang-2 and SBP in the patients of Hunt-Hess I～III were significantly lower than them of Hunt-Hess IV～V (Z=-3.928, P=0.000; Z=-3.657, P=0.000；Z=-2.127, P=0.034). Conclusions: These results suggest that serum Ang1 and Ang2 levels are promising clinically informative biomarkers of disease severity in aSAH.

Key words: angiopoietins, human intracranial aneurysms, aSAH , Hunt-Hess, Hypertension, serum, ELISA