天津医药

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IL-23p19mRNA在RA患者滑膜组织及滑膜细胞中的表达

赵文君1,陆芸2,张凯2,于顺禄3,郭刚4,邢国胜3,魏学磊3   

  1. 1. 天津市天津医院骨科研究所
    2. 天津市天津医院
    3. 天津医院
    4. 天津医科大学
  • 收稿日期:2010-07-16 修回日期:2010-10-20 出版日期:2011-02-15 发布日期:2011-02-15
  • 通讯作者: 赵文君

The expression of IL-23p19mRNA in RA synovial tissues and RA synovial fibroblasts

  • Received:2010-07-16 Revised:2010-10-20 Published:2011-02-15 Online:2011-02-15

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摘要： 目的：探讨类风湿性关节炎(RA)与骨性关节炎(OA)患者滑膜组织及体外培养滑膜成纤维细胞(SF)中IL-23p19mRNA分泌来源及对RA诊断的意义，初步探讨其在RA发病中的作用方式。方法：收集天津医院关节科行滑膜切除术及关节置换术的6例RA患者滑膜组织及6例OA患者滑膜组织，并采用酶消化法对上述滑膜组织行SF体外培养，采用RT-PCR方法检测上述RA和OA滑膜组织及SF中IL-23p19mRNA表达水平，RA患者滑膜组织及体外培养SF中IL-1βmRNA的表达水平。结果：①6例RA患者滑膜组织及SF中IL-23p19mRNA表达均为阳性，4例OA患者滑膜组织及OASF中IL-23p19mRNA表达阳性，RA患者滑膜组织中IL-23p19mRNA的表达显著高于OA患者滑膜组织(p<0.05)，RASF中IL-23p19mRNA的表达量显著高于OASF中阳性标本的表达量(p<0.01)。②6例RA患者滑膜组织中IL-1βmRNA表达均阳性，且其表达水平与IL-23p19mRNA表达水平有明显正相关性(r=0.900 ，p<0.05)，6例RASF中IL-1βmRNA表达均阳性，且其表达水平与IL-23p19mRNA表达水平有明显正相关性(r=0.883，p<0.05)。结论：IL-23参与了RA的发病过程，并且RA患者中SF也参与分泌，IL-23可作为RA与OA鉴别诊断的指标之一。RA患者滑膜组织与SF中IL-23p19mRNA表达水平与IL-1βmRNA呈正相关，IL-1β很可能是IL-23在RA中发挥致病作用的通路之一。

关键词: 类风湿性关节炎, 骨性关节炎, 细胞因子, 滑膜成纤维细胞, IL-23

Abstract: Abstract Objective: Discuss the secretion source and the diagnostic of IL-23 in RA patients, find out the pathway of IL-23 involved in RA pathogenesis. Methods: 6 Synovial tissues of RA and 6 Synovial tissues of OA were obtained during synovectomy and joint replacement surgery. RASF and OASF was extracted by enzyme from synovial. RT-PCR was used to detected the IL-23p19mRNA, IL-1βmRNA and GAPDH mRNA. Results: ①IL-23p19mRNA is positive in all of the 6 RA synovial tissues and 6 RASF, 4 OA synovial tissues and 4 OASF is positive. In all the positive samples, RA is significant higher than OA (p<0.05). ② All of the 6 RA synovial tissues is positive in the expression of IL-1βmRNA, which is correlated to the expression of IL-23p19mRNA(r=0.900, p<0.05), All of the 6 RASF is positive in the expression of IL-1βmRNA, and it is also correlated to the expression of IL-23p19mRNA in RASF(r=0.883, p<0.05). Conclusion: IL-23 is involved in the pathogenesis of RA, SF can secrete IL-23. IL-23 may be a verify of RA and OA. The expression of IL-23p19mRNA in RA synovial tissues and RASF is correlated with IL-1βmRNA illustrate that IL-1β maybe a pathway of IL-23 involved in RA pathogenesis.

Key words: rheumatoid arthritis, osteoarthritis, cytokine, synovial fibroblast, IL-23