天津医药

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胶滴药敏试验在不同分化胃癌细胞株上的运用

张建良1,谭志军2,姜伟2   

  1. 1. 天津医科大学
    2. 天津市第一中心医院
  • 收稿日期:2010-07-26 修回日期:2010-12-11 出版日期:2011-03-15 发布日期:2011-03-15
  • 通讯作者: 谭志军

The use of collagen gel droplet culture drug sensitivity test in different gastric cancer cell lines

  • Received:2010-07-26 Revised:2010-12-11 Published:2011-03-15 Online:2011-03-15

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摘要： 摘要 目的：利用胶滴药敏实验来评估化疗药物对人胃中分化腺癌细胞株SGC-7901、人胃低分化腺癌细胞株BGC-823、人胃粘液腺癌细胞株MGC-803的敏感性。方法：利用三个胃癌细胞株SGC-7901 BGC-823 MGC-803建立胶滴药敏试验，每个细胞株用顺铂、奥沙利铂、5-FU、依托泊苷、阿霉素、丝裂霉素六种化疗药物分别设置25﹪、50﹪、100﹪、200﹪、400﹪五个浓度梯度测定胃癌细胞的敏感性和抑制率。结果：六种化疗药顺铂、奥沙利铂、5-FU、依托泊苷、阿霉素、丝裂霉素在标准浓度即100%测试药物浓度（100%TDC）下对人胃粘液腺癌细胞株MGC-803的抑制率分别为58.68、63.85、57.60、28.09、23.18、40.18，对人胃低分化腺癌细胞株BGC-823的抑制率分别为59.09、61.26、56.30、33.21、33.26、41.20,对胃中分化腺癌细胞株SGC-7901的抑制率分别为61.25、69.76、60.78、35.00、30.08、58.80。结论：CD-DST体外胶滴药敏试验是一种较好的药敏检测方法，对化疗药物的体外筛选和个体化治疗具有实际应用价值。

关键词: 体外药敏试验, 胶滴药敏试验（CD-DST）, 胃癌

Abstract: Abstract Objective:Use the collagen gel droplet culture drug-sensitivity to assess its inhibition rate of different gastric cancer cell lines.Methods:Three gastric cancer cell lines with SGC7901 BGC823 MGC803 established dispensing drug sensitivity test, each cell line were set up with five of six chemotherapy drug concentration gradient measured the sensitivity of cancer cells. Results:Six chemotherapy drug in the standard concentration of human gastric mucous adenocarcinoma cell line MGC-803 inhibition rates were 58.68、63.85、57.60、28.09、23.18、40.18，poorly differentiated human gastric adenocarcinoma cell line BGC-823 inhibition rates were 59.09、61.26、56.30、33.21、33.26、41.20, differentiated adenocarcinoma of the stomach cell line SGC-7901 were 61.25、69.76、60.78、35.00、30.08、58.80。Conclusion ：CD-DST dispensing sensitivity test is a better drug susceptibility testing method, it is useful in vitro anticancer drug screening and individualized treatment practical application.

Key words: Collagen gel droplet culture drug sensitivity test (CD-DST), chemosensitivity test, Gastric cancer