天津医药

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芳香化酶抑制剂对子宫内膜异位症大鼠异位灶中生存素及Caspase-3的影响

齐之迎1,尹利荣2,马洪达1,宋淑芳3   

  1. 1. 天津医科大学第二医院
    2. 天津医科大学第二医院妇科
    3. 天津医科大学第二医院妇科；内蒙古医学院第一附属医院妇产科
  • 收稿日期:2010-05-10 修回日期:2010-11-19 出版日期:2011-03-15 发布日期:2011-03-15
  • 通讯作者: 齐之迎

The effects of aromatase inhibitors on expressions of Survivin and Caspase-3 in the ectopic focus of rat model endometriosis

  • Received:2010-05-10 Revised:2010-11-19 Published:2011-03-15 Online:2011-03-15

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摘要： 【摘要】目的：通过建立大鼠子宫内膜异位症模型，探讨芳香化酶抑制剂对异位灶中survivin及caspase-3表达的影响。方法：采用自体子宫内膜移植法建立大鼠子宫内膜异位症模型，造模3周后开腹观察及测量异位灶形态及大小，计算体积，并将模型组大鼠随机分为来曲唑组及生理盐水（NS）对照组，每组20只。给药4周后处死大鼠，观察及测量这两组异位灶形态及大小；镜下观察这两组及只行开关腹手术（假手术）组的大鼠正常子宫内膜的组织学变化，并采用免疫组化法检测两组异位内膜及正常内膜组织的survivin及caspase-3表达情况。结果：成功建立大鼠子宫内膜异位症模型，给药4周后，来曲唑组异位灶大小较给药前减小，差异有统计学意义(P<0.05)；survivin及caspase-3均在子宫内膜腺上皮细胞表达，定位于细胞浆。survivin在来曲唑组及正常子宫内膜组的阳性率均低于NS对照组，差异均有统计学意义(P<0.01)，而survivin在来曲唑组与正常内膜组阳性率无差异（P>0.01）; caspase-3在来曲唑组及正常子宫内膜组的阳性率均高于NS对照组，差异均有统计学意义(P<0.01), 而caspase-3在来曲唑组与正常内膜组阳性率无差异（P>0.01）。survivin与caspase-3的表达呈负相关（r=-0.538，P<0.05）。结论：芳香化酶抑制剂可抑制大鼠子宫内膜异位症病灶的生长，并抑制异位灶中survivin的表达，增加caspase-3的表达，为子宫内膜异位症的临床治疗奠定理论基础。

关键词: 子宫内膜异位症, 大鼠, 芳香化酶抑制剂, survivin, caspase-3

Abstract: 【Abstract】 Objective: To explore effect of aromatase inhibitor on the expression of survivin and Caspase-3 in ectopic focus through establishing rat model of endometriosis . Methods: Rat model of endometriosis was made by way of autologous transplantation of endometrium and 3 weeks later we observed and measured the shape and size of the ectopic foci in abdominal cavity，calculation of volume. Rats were randomly divided into two groups ( twenty in each): Letrozole group and physical saline group as control（NS）. After 4 weeks of administration we observed endometrial histological changes of the two groups and normal rats which were only imposed to abdominal switches surgery without transplantation .Expression level of survivin and Caspase-3 were assessed by immunohistochemical staining in ectopic endometrium and normal endometrium. Results: we successfully established the rat model of endometriosis.After 4 weeks of administratio ,the size of ectopic focus in Letrozole group was reduced and the differences were statistically significant (P <0.05);and both survivin and Caspase-3 were expressed in endometrial glandular epithelial cells, locating in cytoplasm. Results showed that positive rate of survivin in the letrozole group and the normal endometrium group were lower than saline control group and the differences were statistically significant (P <0.01) while there was no difference between the letrozole group and normal endometrial cells (P>0.01); Positive rates of caspase-3 in the letrozole group and the normal endometrium group were higher than saline control group and the differences were statistically significant (P <0.01), while there was no difference between the letrozole group and normal endometrial cells (P> 0.01); The expression of survivin is negatively correlated with that of Caspase-3（r=-0.538，P<0.05）.Conclusion: The aromatase inhibitor can inhibit the growth of ectopic focus and survivin expression and increase expression of caspase-3 in rat ectopic endometrial lesions .The results may offer theoretical foundation for clinical application of letrozole in treatment of endometriosis .

Key words: endometriosis, aromatase inhibitor, rat, survivin, caspase-3