天津医药

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β-磷酸三钙表面吸附P-15多肽对细胞相容性的影响

赵军1,沈文文2,3,樊洪1   

  1. 1. 天津市口腔医院
    2.
    3. 南开大学医学院
  • 收稿日期:2010-09-13 修回日期:2010-11-23 出版日期:2011-05-15 发布日期:2011-05-15
  • 通讯作者: 赵军

Effect of P-15 Peptide Attachedβ- Tricalcium Phosphate Surface on Cellular Compatibility

  • Received:2010-09-13 Revised:2010-11-23 Published:2011-05-15 Online:2011-05-15

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摘要： 【摘要】目的：在体外研究β-磷酸三钙（β-tricalcium phosphate，β-TCP）吸附15肽（P-15）后对细胞相容性的影响。方法：在吸附有P-15多肽的β-TCP和单纯的β-TCP上培养骨形态发生蛋白2(Bone morphogenetic protein 2,BMP2)诱导的鼠源的成肌细胞系C2C12，分别于培养的第2，4和6天取材，CCK8检测细胞增殖情况； BCA法检测蛋白质含量；碱性磷酸酶（alkaline phosphatase，ALP）试剂盒检测ALP活性。结果：吸附P-15多肽的β-TCP上的细胞增殖情况、碱性磷酸酶活性和蛋白质含量均明显高于未吸附P-15多肽组。结论：β-TCP吸附P-15后提高了其生物相容性。

关键词: β-磷酸三钙, P-15, 细胞相容性 , 成肌细胞 , 骨形态发生蛋白2

Abstract: 【Abstract】 Objective: Evaluate the effect of P-15 peptide attachedβ- tricalcium phosphate (β-TCP )surface on cellular compatibility in vitro. Methods: myoblast C2C12 treated by BMP2 was cultured on the surface ofβ-TCP/P-15 and β-TCP, samples were obtained 2、4、6 days after culture.Cell proliferation was detected by CCK8 method;Cell alkaline phosphatase activities were assayed by ALP kit;Total cell protein was examined by BCA method. Results: The cell proliferation、ALP activity and total amount of protein of C2C12 adhered to β-TCP attched by P-15 peptide were obviously higher than that of C2C12 adhered toβ-TCP Conclusion: β-TCP surface attached P-15 improve its biocompatibility.

Key words: β-TCP, P-15, Cellular compatibility, Myoblasts , BMP2