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HIF-1α和STAT3分子表达与大肠癌淋巴转移的关系

朱凤池1,戚峰2,巩发才2   

  1. 1. 天津医科大学总医院普外科
    2. 天津医科大学总医院
  • 收稿日期:2010-12-08 修回日期:2011-03-28 出版日期:2011-08-15 发布日期:2011-08-15
  • 通讯作者: 朱凤池

The Relationship between the Expression of HIF-1α and STAT3 Molecules and Lymphatic Metastasis of Colorectal Carcinoma.

  1. 1. 戚峰
    2.
  • Received:2010-12-08 Revised:2011-03-28 Published:2011-08-15 Online:2011-08-15

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2016

摘要： 摘要 目的：探讨缺氧诱导因子（HIF-1α）、信号转导和转录活化因子３(STAT3)在大肠癌淋巴转移中的作用机制。方法：采用荧光实时定量PCR和免疫组织化学染色方法检测60例大肠癌组织及远癌切端大肠癌组织HIF-1α、STAT3和VEGF-Ｃ的mRNA转录水平和蛋白表达情况，同时以抗podoplanin单克隆抗体标记大肠癌微淋巴管计数淋巴管密度（LVD），分析上述指标与大肠癌淋巴结转移之间的相互关系。结果：大肠癌组织中HIF-1α、STAT3和VEGF-C分子的mRNA转录水平及蛋白表达水平明显高于远癌切端大肠癌组织（P＜0.05），大肠癌组织中HIF-1α、STAT3与VEGF-C分子mRNA转录水平及蛋白表达水平呈正相关，有淋巴结转移患者LVD高于无淋巴结转移患者（P＜0.05）。结论：大肠癌组织较远癌切端大肠组织有更为密集的淋巴系统，大肠癌组织中HIF-1α、STAT3、VEGF-C是调节大肠癌淋巴管形成的关键因子。

关键词: 大肠癌, 微淋巴管, HIF-1α, STAT3, VEGF-C, podoplanin

Abstract: ABSTRACT Objective: Study the mechanism of action of hypoxia inducible factor-1α(HIF-1α), signal transducers and activators of transcription 3(STAT3) in lymphatic metastasis of colorectal carcinoma. Methods: The levels of HIF-1α, STAT3 and VEGF-Ｃ mRNA transcription and protein expression of adenocarcinoma and surgical margin tissues gotten from 60 cases with CRC were determined by real-time quantitative PCR using fluorescence and immunohistochemistry, as well as lymph vessel density was counted using anti-podoplanin monoclonal antibody. And correlations of above indexes and lymphatic metastasis of colorectal carcinoma were analyzed. Results: The levels of HIF-1α, STAT3 and VEGF-Ｃ of tumor tissues were significant higher than that of surgical margin in either mRNA transcription or protein expression(P＜0.05). The levels of HIF-1α, STAT3 and VEGF-Ｃ of tumor tissues were more significant relative not only in either mRNA transcription or protein expression but also in lymphatic metastasis group(P＜0.05). Conclusion: The expression of lymphatic vessels system in colorectal carcinoma is higher than that in surgical marginal tissue. HIF-1α,STAT3 and VEGF-C are the key regulators in lymphatic vessel formation of colorectal carcinoma,and are closely related to lymphatic metastasis.

Key words: colorectal carcinoma, microlymphatics, HIF-1α, STAT3, VEGF-C, podoplanin