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葛根素联合牛磺酸对2型糖尿病大鼠胰腺的保护作用

门秀丽1,赵利军2,孔小燕1,赵霞1,李克宁1,张连元1   

  1. 1. 河北联合大学基础医学院病理生理学教研室
    2. 河北省唐山市华北煤炭医学院
  • 收稿日期:2011-02-21 修回日期:2011-04-07 出版日期:2011-08-15 发布日期:2011-08-15
  • 通讯作者: 门秀丽

Protective Effects of Puerarin Combined with Taurine on Pncreas of Rats with Type 2 Diabetes

  • Received:2011-02-21 Revised:2011-04-07 Published:2011-08-15 Online:2011-08-15

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摘要： 目的 探讨葛根素联合牛磺酸对2型糖尿病大鼠胰腺的保护作用。方法 将40只Wistar大鼠随机分为糖尿病组 (DM组 )、单用牛磺酸组（Tau组）、单用葛根素组（Pue组）和葛根素联合牛磺酸组 (Pue +Tau组)。8周后, 测4组大鼠糖化血红蛋白、血浆葡萄糖、胰岛素、超氧化物岐化酶（SOD）、丙二醛 (MDA)、活性氧(ROS)及胰腺线粒体MDA、ROS及SOD，免疫组织化学方法检测各组大鼠胰腺组织PDX-1基因的表达。结果 与糖尿病组相比，单独应用牛磺酸或葛根素均能不同程度地降低糖尿病大鼠血糖、糖化血红蛋白和血浆及胰腺线粒体MDA和ROS水平，升高造模大鼠的SOD活性及血浆胰岛素水平；并且免疫组织化学染色结果显示PDX-1基因表达上调；而这些变化在葛根素联合牛磺酸组更加明显。结论 葛根素联合牛磺酸对2型糖尿病大鼠胰腺有保护作用，其机制可能与降低氧化应激水平，上调PDX-1基因表达有关。

关键词: 牛磺酸, 葛根素, 2型糖尿病, PDX-1

Abstract: 　Ojective　To investigate the effects of puerarin combined with taurine on pancreas of rats with type 2 diabetes. Methods　40 rats were divided into diabetes mellitus group (DM), Taurine treatment group(Tau), Puerarin treatment group(Pue) and combined with taurine treatment group(Pue+Tau) at random. After having been treated with puerarin or/and taurine for 8 weeks, Glycosylated Hemoglobin (HbA1c) and the serum contents of glucose, insulin, malondialdehyde (MDA), superoxide dismutase ( SOD) and reactive oxygen species(ROS) were measured; the activity of SOD, MDA and ROS in pancreatic mitochondria were detected respectively. Immunohistochemistry technique was used to detect the expression of PDX-1 in pancreas in different group rats respectively. Results Compared with DM group, taurine or puerarin can reduce the content of glucose, MDA, ROS and HbA1c and raise the activity of SOD and the insulin content. The expression of PDX-1 protein was significantly up-regulated in pancreatic islets in Pue+Tau group compared with that of DM group. Conclusion These results suggest that puerarin combined with taurine could prevent the pancreas from oxidative stress in diabetic rats and induced the up-regulation of PDX-1 expression.

Key words: 　Taurine, puerarin, Type 2 diabetes mellitus, PDX-1