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地塞米松诱导乳腺癌细胞对紫杉醇耐药的研究

丁凌雁   

  1. 湖南省永州市中心医院南院（永州市零陵区潇湘西路151号）
  • 收稿日期:2010-10-09 修回日期:2011-02-27 出版日期:2011-09-15 发布日期:2011-09-15
  • 通讯作者: 丁凌雁

The study of Glucocorticoid-induced inhibition of chemotherapy to paclitaxel in breast carcinomas

ding ling yan   

  • Received:2010-10-09 Revised:2011-02-27 Published:2011-09-15 Online:2011-09-15
  • Contact: ding ling yan

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摘要： 【目的】观察不同浓度地塞米松预处理人乳腺腺癌细胞后再予紫杉醇干预，乳腺腺癌细胞耐药发生概率及细胞中细胞分裂素活化蛋白激酶磷酸酯酶-1（MKP-1）mRNA表达的变化。【方法】四唑盐(MTT) 试验测定紫杉醇对乳腺腺癌细胞MCF-7 的抑制率, 筛选出药物的IC50浓度。将不同浓度的地塞米松预处理乳腺腺癌细胞MCF-7，再予紫杉醇(PTX) 作用于乳腺腺癌细胞MCF-7，四唑盐(MTT)试验测定细胞存活率及RT-PCR法检测细胞中细胞分裂素活化蛋白激酶磷酸酯酶-1（MKP-1）mRNA的表达水平。【结果】不同浓度的地塞米松预处理乳腺癌细胞MCF-7后能诱导其对紫杉醇产生耐药，耐药的概率随着糖皮质激素浓度的增加而增加，细胞分裂素活化蛋白激酶磷酸酯酶-1（MKP-1）mRNA的表达水平随着糖皮质激素浓度的增加而增加 ( P < 0.01)。【结论】糖皮质激素预处理乳腺腺癌细胞MCF-7后能诱导其对紫杉醇产生耐药， 其机制可能与MKP-1抑制细胞凋亡而介导乳腺癌耐药有关。

关键词: 糖皮质激素, 乳腺腺癌细胞株, 紫杉醇, 耐药, MKP-1

Abstract: Abstract：【Objective】To observe the changes of the probability of inhibition and the mitogen-activated protein kinase phosphatase-1(MKP-1)expression in human breast adenocarcinomal cell after dexamethasone pretreatment with difficult concentration and treatment of paclitaxel. 【Methods】MTT assay (mononuclear cell direct cytoxicity assay) : to estimate the survival rate of paclitaxel on breast adenocarcinomal cell MCF-7 , screening the concentration of IC50 in this medicine. To place different concentration of dexamethasone pretreatmently and paclitaxelsuccedently on MCF-7 cell，MTT assay to estimate the survival rate of paclitaxel on MCF-7 cell and reverse transcriptase-polymerase chain reaction(RT-PCR)to detect the expression level of the mitogen—activated protein kinase phosphatase-1 （MKP-1）mRNA in MCF-7 cell( P < 0.05). 【Results】After being pretreatmented with dexamethasone with difficult concentration，MCF-7 cell was induced inhibition of chemotherapy to paclitaxel，the inhibition rate increased gradually with the increasing concentration of dexamethasone and the expression level of MKP-1 mRNA increased gradually with the increasing concentration of dexamethasone also. 【Conclusions】After being pretreatmented with dexamethasone with difficult concentration，MCF-7 cell was induced inhibition of chemotherapy to paclitaxel，which make clear that MKP-1 may be involved in the path-ways of chemotherapy inhibition to breast carcinomas through resistance cellular apoptosis.

Key words: Glucocorticoid, Breast adenocarcinomal cell strain, Paclitaxel, Inhibition, MKP-1