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雄激素对前列腺癌细胞系PIP表达的影响

崔书霞,郝志梅,郭志义,郝小惠,王素云,田炜   

  1. 河北联合大学
  • 收稿日期:2011-09-27 修回日期:2012-05-24 出版日期:2012-11-15 发布日期:2012-11-15
  • 通讯作者: 田炜

Effects of androgen on PIP mRNA expression in human prostate cancer cell line

  • Received:2011-09-27 Revised:2012-05-24 Published:2012-11-15 Online:2012-11-15
  • Contact: Wei TIAN

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摘要： 目的：探讨PIP在前列腺癌不同细胞系中的表达情况，以及雄激素对其表达的影响。方法：应用不同浓度（0、0.1、1、10、100 nmol/L）双氢睾酮（Dihydrotestosterone，DHT）或睾酮（Testosterone，T）及不同时间（6、24和48 h）对前列腺癌细胞系（LNCaP、PC-3、DU145）进行处理，通过RT-PCR及实时荧光定量PCR法检测PIP 及AR mRNA表达情况。通过Western blot法检测各细胞系（LNCaP、PC-3、DU145、T47D）中PIP蛋白表达情况。结果：实时荧光定量RT-PCR法与Western blot结果显示，雄激素依赖性前列腺癌细胞系LNCaP中有PIP mRNA及蛋白表达，PIP mRNA的表达随DHT或T浓度增加而增加，且于10 nmol/L浓度DHT或T时达到最大峰值。而AR mRNA的表达差异无统计学意义。雄激素非依赖性前列腺癌细胞系PC-3与DU145中无PIP mRNA及蛋白表达，并且AR表达较少或无AR表达。结论：PIP或许在前列腺癌发生发展中，以及鉴别前列腺癌是否具有雄激素依赖性方面有一定作用。

关键词: 前列腺癌, 雄激素, PIP, 实时荧光定量PCR

Abstract: Objective: The purpose of this study was to determine prolactin-inducible protein (PIP) mRNA expression, and effects of androgen in human prostate cancer cell lines. Methods: By different concentrations and different times of DHT or T, PIP and AR mRNA expression in prostate and breast tumor cell lines (LNCaP, PC-3, DU145 and T47D) were determined by RT-PCR and FQ RT-PCR. PIP protein expression in each cell lines was determined by Western blot. Results: By RT-PCR and western blot, PIP mRNA and protein expression was detected in androgen-dependent PCa cell line LNCaP. PIP mRNA expression was increase along with concentration of DHT or T in LNCaP. PIP mRNA level reach maximum peak in 10 nmol/L DHT or T. AR mRNA expression was unchanged treated by different concentration of DHT or T in LNCaP cell lines. Conclusions: The role of PIP may occur in the process of development of prostate cancer, and differentialdiagnosis prostate cancer whether or not androgen dependence.

Key words: prostate cancer, androgen, prolactin-inducible protein, real-time fluorescent quantitative RT-PCR