天津医药

• 实验研究 • 上一篇    下一篇

凉膈散对肠缺血再灌注损伤大鼠肠道的保护作用

常文秀1,曹书华2,王勇强3,杨书英1   

  1. 1. 天津市第一中心医院
    2. 天津市第一中心医院ICU
    3. 天津市第一中心医院急救研究所
  • 收稿日期:2011-03-09 修回日期:2011-05-30 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 常文秀

The protective effect of Liangge San on intestinal I/R injury

  • Received:2011-03-09 Revised:2011-05-30 Published:2012-02-15 Online:2012-02-15

PDF (PC)

2025

摘要： 目的：观察凉膈散对大鼠缺血再灌注（I/R）损伤肠黏膜的保护作用。方法：健康Wistar大鼠168只，随机分为正常组、假手术组、模型组、凉膈散治疗组和凉膈散防治组，其中正常组8只，其他4组各40只。正常组不予任何处理，其余4组通过夹闭肠系膜上动脉建立大鼠小肠I/R模型，根据缺血45 min后再灌注3、12、24、48和72 h 5个时相点又分为5小组，每小组8只大鼠。测定各组5个时点动物小肠黏膜病理损伤Chiu氏评分、门静脉血浆D-乳酸值、小肠组织匀浆MDA含量和SOD活力、肠黏膜凋亡指数及Bax表达水平。结果：模型组动物肠黏膜结构破坏明显，Chiu氏评分、血浆D-乳酸值明显升高。小肠匀浆MDA含量升高、SOD活力降低，肠黏膜细胞凋亡指数及Bax表达水平升高。凉膈散干预后，可减轻小肠黏膜病理损伤程度，与模型组比较，除3 h外，门静脉血浆D-乳酸值下降，小肠组织匀浆MDA含量降低和SOD活力升高、肠黏膜凋亡指数及Bax表达水平均下降。结论：I/R损伤可破坏肠黏膜屏障功能，凉膈散可通过减少机体氧自由基的生成而保护肠黏膜。

关键词: 凉膈散, 再灌注损伤, 肠黏膜, 乳酸, 丙二醛, 超氧化物歧化酶, 细胞凋亡

Abstract: Objective: To observe the protective effect of Liangge San on intestinal I/R injury. Method: A total of 168 Wistar rats were randomly divided into 5 group: normal group, sham operation group, model group, Liangge San therapeutics group and Liangge San prevention and cure group. The I/R model was made by blocking the superior mesenteric artery, and Liangge San was used to intervene. Chiu’s score, D-lactic acid content of janitrix blood plasma, MDA content and SOD activity of intestinal homogenate, apoptotic intex, the expression of the apoptotic protein Bax in the small intestine cells were measured after 45 min of ischemic and 3h、12h、24h、48h、72h after reperfusion. Results: After I/R, the intestinal morphology was significantly injuried, Chiu’s score and D-lactic acid contend of blood rised. The intestinal MDA content in model group increased and SOD activity decreased. The apoptotic index and the expression of Bax in intestinal mucosal cells of model group were obviously higher than those in other gropps. After the intervention of Liangge San, Chiu’s score and D-lactic acid content were lower than those in model group. MDA content decreased and SOD activity increased. The apoptotic index and the expression of Bax all dropped. Conclusions: Schemic reperfusion injury can destroy the function of intestinal barrier, Liangge San can protect the intestinal mucosa through cleaning the oxygen free radical.