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外阴阴道假丝酵母菌病与抗菌肽LL-37及防御素5的相关性研究

李红1,尹利荣2,王芳1   

  1. 1. 天津医科大学第二医院
    2. 天津医科大学第二医院妇科
  • 收稿日期:2011-06-30 修回日期:2011-10-08 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 尹利荣

Study on the Correlation of the Vulvovaginal Candidiasis（VVC）with Antibiotic Peptide LL-37and HD5

1,   

  • Received:2011-06-30 Revised:2011-10-08 Published:2012-02-15 Online:2012-02-15

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摘要： 摘要 目的：检测外阴阴道假丝酵母菌病（Vulvovaginal Candidiasis，VVC）及复发性外阴阴道假丝酵母菌病（Recurrent Vulvovaginal Candidiasis，RVVC)患者阴道分泌物中抗菌肽LL-37和防御素5（Human Defensin 5，HD5）的表达及意义。 方法：收集VVC患者31例、RVVC患者27例及正常对照组30例，应用科玛嘉念珠菌显色培养基筛选出白假丝酵母菌感染病例，采用酶联免疫吸附测定法(ELISA)测定其阴道分泌物中抗菌肽LL-37及HD5的含量。结果： LL-37及HD-5在VVC组、RVVC组及正常对照组均有表达； VVC组LL-37、HD5水平较正常对照组均显著升高，差异有统计学意义（P<0.05）；RVVC组LL-37水平较正常对照组升高，差异有统计学意义（P<0.05）；RVVC组HD5水平与正常对照组相比，差异无统计学意义（P>0.05）；VVC组LL-37、HD5 水平与RVVC组相比，差异无统计学意义（P>0.05）。 结论： 抗菌肽LL-37与VVC发病及RVVC反复发作有关；HD5与VVC发病有关，但与RVVC复发关系不确切。

关键词: 外阴阴道假丝酵母菌病, 抗菌肽LL-37, HD5, 相关性

Abstract: Abstract Objective: To investigate the expression of Antibiotic Peptide LL-37and HD5 in the vaginal fluid of vulvovaginal candidiasis（VVC）and recurrent vulvovaginal candidiasis(RVVC). Methods：Human vaginal fluid from 31 VVC patients, 27 RVVC patients and 30 healthy women ( the control group)were collected respectively.Enzyme-linked immunosorbent assay ( ELISA)was applied to detect the levels of antibiotic Peptide LL-37and HD5 which were infectded by candida albicans that were found by culturing in the CHROMagar. Results: Both the LL-37and HD5 were found in the VVC group、RVVC group and the control group. The levels of LL-37and HD5 of VVC group were higher than the control group.The level of LL-37 of RVVC group was higher than the control group. The level of HD5 had no difference between the RVVC group and the control group. The levels of LL-37 and HD5 had no difference between the RVVC group and the VVC group. Conclusions: The level of LL-37 may play an important role in the pathogenesis of VVC and RVVC. The level of HD5 may play an important role in the pathogenesis of VVC , while may had no relationship with RVVC.

Key words: Vulvovaginal candidiasis, Antibiotic Peptide LL-37, HD5, Correlation