天津医药

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恩度联合放射对鼻咽癌裸鼠成瘤的抑制作用及机制

徐晓婷,李金利,秦颂兵,周菊英   

  1. 苏州大学附属第一医院放疗科
  • 收稿日期:2011-10-21 修回日期:2011-12-19 出版日期:2012-05-15 发布日期:2012-05-15
  • 通讯作者: 李金利

The study of irradion combined with Endostar therapy for human nasopharyngeal carcinoma xenografts in nuke mice

  • Received:2011-10-21 Revised:2011-12-19 Published:2012-05-15 Online:2012-05-15

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摘要： 目的：观察重组人血管内皮抑素（恩度）联合放射对鼻咽癌裸鼠成瘤的抑制作用及对VEGF和Survivin mRNA表达的影响。方法：建立鼻咽癌裸鼠成瘤模型，成瘤后随机分为4组，对照组(A)、恩度组(B)、放射组(C)和恩度+放射联合组(D)，观察各组移植瘤的生长速度和瘤体大小的变化。4周后处死裸鼠摘取瘤体，电镜观察各组肿瘤凋亡情况，应用RT-PCR检测各组瘤体组织中的VECF和Survivin mRNA的表达变化。结果：联合组对鼻咽癌移植瘤的生长有显著抑制作用，且效果明显优于其它三组，差别有统计学意义（t=32.454,P<0.01; t=25.398,P<0.01; t=7.754,P<0.05）；电镜下观察联合组凋亡率明显高于其它三组，差别有统计学意义（t=21.651,P<0.01; t=15.431,P<0.01; t=10.832,P<0.01）。联合组中VECF和Survivin的表达与另外三组相比均显著下调，VEGF与Survivin的表达呈正相关（r=0.679，P＜0.01）。结论：恩度联合放射能明显抑制鼻咽癌裸鼠移植瘤的生长，这种作用可能与VEGF和Survivin表达下调有关。

关键词: 重组人血管内皮抑制素, 鼻咽恶性肿瘤, 放射敏感性, 血管内皮生长因子

Abstract: Objective: To observe the inhibitory effect of Endostar combined with radiotherapy on transplanted CNE1 Nasopharyngeal Carcinoma(NPC) in BALB/c nude mice and explore the relationship of this inhibitory effect with the expression level of VEGF and apoptosis inhibiting protein-Survivin..Methods: The transplanted tumor model of human CNE1 nasopharyngeal carcinoma on nude mice was established to evaluate the antitumor activity of Endostar combined with radiation (Endostar+radiation,D group) treatment compared with monotherapy (Endostar,B group or radiation,C group).The growth characteristics of transplanted tumor were recorded. Transmission electron microscopy were used to observe the morphological changes of cell apoptosis. Real-time quantitative PCR was used to determine the effect on VEGF and Survivin mRNA expression level of of tumor tissues.Results: Significant on the tumor growth was found ,and more typical morphological changes were seen in cancer cells in D group. Level of VEGF in tumor tissue of D group was lower than that of C group ,B group or A group(P<0.05). And the level of Survivin in D group were much lower than in other three groups(P<0.05). The results of linear Correlation relevant analysis showed that there exist positive correlation between the inhibitory effect and the expression of VEGF and Survivin (r = 0.679, P < 0.01).Conclusion: Endostar could enhance the radiosensitivity of NPC transplantation tumor in nude mice to radiation.The mechanism may be down-regulating the expression of VEGF and Survivin.

Key words: Endostar, Nasopharyngeal Carcinoma, radiosensitivity, VEGF