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依达拉奉对脑出血大鼠血肿周围组织caspase3和pdcd5蛋白表达的影响

赵彬1,刘斌2   

  1. 1. 河北联合大学校医院
    2. 河北联合大学附属医院
  • 收稿日期:2011-07-11 修回日期:2011-10-10 出版日期:2012-05-15 发布日期:2012-05-15
  • 通讯作者: 刘斌

Effect of Edaravone on expression of caspase3 and pdcd5 protein in intracerebral hemorrhage rat

  • Received:2011-07-11 Revised:2011-10-10 Published:2012-05-15 Online:2012-05-15
  • Contact: liu bin

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摘要： 目的 探讨依达拉奉对脑出血大鼠血肿周围组织caspase3和pdcd5蛋白表达的影响。方法 108只健康雄性Wistar大鼠，随机分为正常组、假手术组、脑出血组和依达拉奉治疗组。后2组又分为3h、6h、12h、1d、2d、3d、5d、7d 8个亚组，每组6只。采用大鼠尾动脉自体不凝血 50μl注入尾状核区建立脑出血模型。免疫组化法检测caspase3和pdcd5蛋白表达变化。结果 与脑出血组相比，依达拉奉治疗组于出血后 6h、12h、1d、2d、3d、5d、7d血肿周围组织caspase3和pdcd5蛋白表达均减少（p<0.05，p<0.01）。 结论 依达拉奉可抑制caspase3和pdcd5蛋白表达，对脑出血后脑组织损伤有保护作用。

关键词: 脑出血, caspase3蛋白, pdcd5蛋白, 依达拉奉

Abstract: Objective To investigate the effect of edaravone on the expression of caspase3 and pdcd5 protein following intracerebral hemorrhage (ICH) in rats. Methods 108 healthy adult Wistar rats were randomized into normal group, sham group ,ICH group,edaravon treat group .The later two groups were randomized into 8 subgroups respectively: 3h, 6h, 12h, 1d，2d，3d，5d，7d groups after ICH,each group 6 rats.ICH models were prepared by injecting 50μl of autogenous blood into the the right caudate nucleus of rats.The expression of caspase3 and pdcd5 protein was observed by immunohistochemistry respectively. Results Compared with hemorrhage group, the expression of caspase3 and pdcd5 protein of Edaravone treat group weakened after 6h、12h、1d、2d、3d、5dand 7d after ICH （p<0.05,p<0.01）). Conclusion The expression of caspase3 and pdcd5 protein following intracerebral hemorrhage were decreased by edaravone .The injury of intracerebral hemorrhage were protected by edaravone.

Key words: Cerebral hemorrhage, Caspase3 protein, Pdcd5 protein, Edaravone