天津医药

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PGA/β-磷酸三钙骨组织工程支架的制备与性能

李欣1,曹宏2,樊洪2   

  1. 1. 天津医科大学
    2. 天津市口腔医院
  • 收稿日期:2011-07-28 修回日期:2011-11-20 出版日期:2012-05-15 发布日期:2012-05-15
  • 通讯作者: 樊洪

Preparation and Propreties of PGA/β- Tricalcium Phosphate of Bone Tissue Engineering Scaffolds

  • Received:2011-07-28 Revised:2011-11-20 Published:2012-05-15 Online:2012-05-15

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摘要： 摘要 目的：制备PGA/β-磷酸三钙(β-TCP)支架并研究其物理性能。方法：以PGA与β-TCP为材料，六氟异丙醇（HFIP）为溶剂，以氯化钠颗粒（颗粒尺寸：425-500μm）为制孔剂，按PGA与β-TCP质量比1：1与1：3，采用改良溶液浇铸-离子沥滤的方法制备PGA/β-TCP骨组织工程复合支架材料。借助扫描电镜对其微结构进行观察，并测试PGA/β-TCP骨组织工程支架复合材料的物理、力学性能。结果： 0.13gPGA/0.39gβ-TCP（B）组支架材料的抗压缩强度大于0.13gPGA/0.13gβ-TCP（A）组，0.13gPGA/0.39gβ-TCP (B)组抗压缩强度值为731.283±55.2140KPa，0.13gPGA/0.13gβ-TCP （A）组抗压缩强度为173.833±36.1397KPa，差异有显著的统计学意义。A、B组支架材料孔隙率均大于85%，A组孔隙率（93.1±0.7%）大于B组（89.3±0.6%），差异有显著的统计学意义。SEM照片可见两组支架材料均为多孔三维架构，孔径均为300-400μm，孔隙的连通性好。β-TCP均匀的分散于聚合物中， B组支架材料中β-TCP颗粒明显多于A组。结论：质量比1：3PGA/β-TCP复合支架材料更满足组织工程对其支架材料的要求。

关键词: 骨组织工程, 支架, 聚羟基乙酸, 复合, β-磷酸三钙

Abstract: Abstract Objective：To fabricate porous PGA/β- Tricalcium Phosphate of tissue engineering scaffolds and test its properties. Methods: Used PGA andβ- Tricalcium Phosphate as materials, 1,1,1,3,3,3-Hexafluoro-2-propanol as solvent, sodium chloride as porogen(particle size :425–500μm), the PGA/β-TCP（in 1：1and 1：3 weight ratios ） scaffolds for tissue engineering are fabricated by solvent-casting particulate leaching and methods. The structure of the scffolds were observed using scanning electron microscopy (SEM). Their properties of physical and mechanics are tested in this paper. Result: The compressive strength of the 0.13gPGA/0.39gβ-TCP（B）group were obviously higher than that the compressive strength of the 0.13gPGA/0.13gβ-TCP（A）group P＜0.001. The compressive strength of the A and B group after surgery were: 731.283±55.2140KPa, 173.833±36.1397KPa. Both A and B groups have a high porosity.(＞85%). The opening porosities of PGA/β-TCP (1:1) scaffolds and PGA/β-TCP (1:3) scaffolds were 93.1±0.7% and 89.3±0.6%, respectively, which is a statistically significant difference . The SEM pictures showed that both scaffolds had irregular micro-pores .The average pore size of two groups were 300-400μm,the β-TCP particles were uniformly dispersed within the PGA matrix.The different between the two groups was that moreβ-TCP granules were present on the pore wall surface of group B compared to group A. Conclusion: The weight ratios (1:3) scaffolds materials can basically meet the requirements of materials for bone tissue engineering.

Key words: bone tissue engineering, scaffolds, polyglycolic acid, composites, β- Tricalcium Phosphate