天津医药

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五味子合剂对糖尿病肾病小鼠肾组织MCP-1及iNOS表达的影响

赵君   

  1. 天津医科大学
  • 收稿日期:2011-10-27 修回日期:2011-12-28 出版日期:2012-06-15 发布日期:2012-06-15
  • 通讯作者: 赵君

Effect of Schisandra Chinensis Mixture on Expression of MCP-1 and iNOS in Renal Tissues of Mice withDiabetic Nephropathy

  • Received:2011-10-27 Revised:2011-12-28 Published:2012-06-15 Online:2012-06-15

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摘要： 摘要 目的：观察五味子合剂(SM)对链脲佐菌素(STZ)诱导的糖尿病肾病(DN)小鼠肾组织MCP-1及iNOS表达水平的影响。方法：雄性C57BL/6 小鼠24 只随机分正常对照组、DN模型组和SM治疗组，采用腹腔注射STZ 法建立DN模型，收集各组血、尿及肾组织，检测血糖、体质量及尿白蛋白肌酐比值(UACR)，光镜观察肾组织形态改变；免疫组化法检测各组肾组织MCP-1及iNOS的表达；RT-PCR检测各组肾组织单核细胞趋化蛋白-1（MCP-1）及诱导型一氧化氮合酶(iNOS)mRNA水平。结果：与模型组比较，SM治疗组血糖及UACR降低；小球系膜区、小管间质区细胞外基质堆积减少。SM能下调肾组织中MCP-1及iNOS的蛋白和mRNA表达水平(P<0.05)。结论：SRC可以通过下调MCP-1及iNOS的表达以减轻肾组织内的炎症反应，从而减轻DN小鼠的肾脏损伤。

关键词: 五味子科, 糖尿病肾病, 单核细胞趋化蛋白-1, 诱导型一氧化氮合酶, 小鼠, 近交C57BL

Abstract: Abstract Objective:To investigate the effect of schisandra chinensis on the expression of MCP-1 and iNOS in renal tissue of mice with Diabetic Nephropathy induced by streptozotocin(STZ). Methods:Twenty-four male C57BL/6 mice were randomly divided into three groups:normal control group,diabetic model group and SM-treated group.The mouse model of diabetes was induced by STZ intraperitoneally.The serum level of glucose,body weight and urinary albumin creatinine ratio(UACR) were measured. Furthermore,the morphological changes of renal tissue were observed under microscope.The expression of mRNA in kidney tissues were determined by RT-PCRl method.The protein expression in kidney tissues were determined by immunohistochemical method. Results:Compared with the diabetic model group,there were significantly lower level of serum glucose and UACR in SM-treated group,SM could also reduce the extracellular matrin accumulation in glomerular mesangial region and renal tubule and interstitial area. There were lower protein and mRNA expression of MCP-1 and iNOS in SM group.,too(P<0.05).Conclusion:After treatment of SM, the expression of MCP-1 and iNOS were decreased, which may reduce renal inflammation and relieve renal injury.

Key words: schisandraceae, diabetic nephropathies, MCP-1, iNOS, mice, C57BL