天津医药

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阿托伐他汀对颅脑损伤大鼠内皮祖细胞和神经功能的影响

金卫篷1,王彬2,赵子龙3,尉辉杰4,5,张永强3,张建宁2   

  1. 1. 天津医科大学第二医院
    2. 天津医科大学总医院
    3. 天津市神经病学研究所
    4.
    5. 天津医科大学总医院神经外科
  • 收稿日期:2012-10-30 修回日期:2012-12-27 出版日期:2013-05-15 发布日期:2013-05-15
  • 通讯作者: 金卫篷

Effects of Atorvastatin on Endothelial Progenitor Cell and Neural Function in Rats with Traumatic Brain Injury

Wei-Peng JIN1,bin wang2,ZHAO Zilong 3,YU Hui jie4,5,ZHANG Yong qiang3,ZHANG JIan ning4   

  1. 1. Department of Neurosurgery，The Second Hospital of Tianjin Medical University
    2. Tianjin Medical University General Hospital
    3. Tianjin Neurology Institute
    4. Department ofNeurosurgery，General Hospital of Tianjin Medical University
    5.
  • Received:2012-10-30 Revised:2012-12-27 Published:2013-05-15 Online:2013-05-15
  • Contact: Wei-Peng JIN

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摘要：

【摘要】 目的  观察阿托伐他汀对颅脑损伤大鼠内皮祖细胞和神经功能的影响。方法 30只成年雄性Wistar大鼠,随机分为假手术组、治疗组和对照组,各10只。治疗组和对照组采用液压打击法制作颅脑损伤模型。假手术组颅骨钻孔后不进行液压打击。治疗组打击后1h口服阿托伐他汀1mg/(kg·d),连续14天。对照组口服等量生理盐水。术后第1、4、7、14、21天测量各组大鼠循环血内皮祖细胞(EPCs)数量,进行mNSS评分。术后第21～25天进行Morris水迷宫实验,记录大鼠目标象限百分率。结果  液压打击后大鼠的神经功能受损。术后第4、7、14、21天,治疗组大鼠循环血EPCs数量高于对照组和假手术组,术后第14、21天治疗组大鼠mNSS评分均低于对照组,术后第24、25天治疗组目标象限百分率显著好于对照组(均P<0.05)。结论  阿托伐他汀能调节颅脑损伤后大鼠循环血EPCs数量,促进大鼠神经功能恢复。 

关键词: 降血脂药, 脑损伤, 干细胞, 内皮细胞, 神经系统, 大鼠, Wistar, 阿托伐他汀

Abstract: [Abstract]  Objective   To observe effects of atorvastatin on endothelial progenitor cells (EPCs) and neural function in rats with traumatic brain injury.  Methods  Thirty adult male Wistar rats were randomly divided into sham group，treatment group and control group，10 for each group. The treatment group and the control group adopted hydraulic combat to produce traumatic brain injury (TBI) model. Rats in sham group underwent the same surgical procedure without being exposed to percussion injury. Rats were treated with atorvastatin (orally, 1 mg·kg-1·d-1) starting 1h after TBI for 14 consecutive days in treatment group. Rats were given saline orally in control group. Circulating EPCs were measured at 1, 4, 7, 14 and 21 days after TBI. The mNSS test was conducted at 1, 4, 7, 14 and 21 days after TBI. Morris water mass（MWM）was performed at 21-25days after TBI. The percentage of target quadrant was recorded in three groups.  Results  The neural function of rats was impaired by hydraulic combat. The mNSS scores were significantly lower in treatment group at 14 and 21 days after TBI compared with those of control group (P < 0.05). The percentage of target quadrant was significantly higher in treatment group than that of control group at 24 and 25 days after TBI (P < 0.05). Conclusion   Atorvastatin can regulate circulating EPCs and promote the recovery of neural function in rats with traumatic brain injury.

Key words: antilipemic agents, brain injury, stem cell, endothelial cell, nervous system, rats, Wistar, 阿托伐他汀