天津医药

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脂多糖诱导持续血小板减少模型的建立及对血小板生成素的影响

姚芳超1,吉祥2,王兵3,王玉亮4,王勇强5,崔尧丽5   

  1. 1. 天津医科大学
    2. 天津医科大学一中心临床学院
    3. 天津市第一中心医院D区ICU
    4. 卫生部危重病急救医学重点实验室
    5. 天津市第一中心医院
  • 收稿日期:2012-07-26 修回日期:2012-12-23 出版日期:2013-05-15 发布日期:2013-05-15
  • 通讯作者: 姚芳超

Establishment of a Continuing Thrombocytopenia Model Induced by Lipopolysaccharide

YAO Fangchao1 1,JI Xiang 1,WANGbing 2,WANG Yuliang 3,WANG Yongqiang 2,CUI Yaoli 2   

  1. 1. Tianjin Medical University
    2. The First Central Hospital of Tianjin
    3. Key Lab of Critical CareMedicine of the Ministry of Health
  • Received:2012-07-26 Revised:2012-12-23 Published:2013-05-15 Online:2013-05-15
  • Contact: YAO Fangchao1

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摘要：

目的  建立脂多糖（LPS）诱导小鼠持续内毒素血症血小板减少（TCP）模型，观察小鼠模型平均血小板体积（MPV）、血小板生成素（TPO）的变化。方法  C57/BL小鼠随机分为4组，低剂量LPS 5 mg/kg连续给药组(LL-c 组),低剂量LPS 5 mg/kg单次给药组（LL组）,高剂量LPS 50 mg/kg单次给药组（HL组），对照组(C组)；于给药前测定各组小鼠血小板计数（PLT）和MPV作为基线，观察给药后小鼠PLT、TPO、MPV水平及存活情况。结果  LL-c组小鼠注射LPS后PLT下降，并持续处于基线30%左右的水平，与基线相比较差异均有统计学意义(P＜0.01)，LL组与HL组均先降低后升高；LL-c组注射LPS后MPV持续处于增大水平，与基线相比较差异有统计学意义(P＜0.01)，HL、LL组MPV均先增大后减小；7 d时LL-c组小鼠TPO高于C组(P＜0.05)；LL-c组、LL组与HL组小鼠造模7 d时分别死亡5只、0只、14只。结论  连续低剂量腹腔注射LPS可诱导小鼠持续TCP模型。

关键词: 脂多糖类, 内毒素血症, 血小板减少, 血小板生成素, 小鼠, 近交C57BL, 平均血小板体积

Abstract: Objective   To establish a model of mouse continuing endotoxemia thrombocytopenia (TCP) induced by lipopolysaccharide (LPS) and observe the change of mean platelet volume (MPV) and thrombopoietin (TPO) in this model. Methods   C57/BL mice were randomly divided into four groups, group of LPS 5mg/kg low dose of continue injection (LL-c group), group of LPS 5mg/kg low dose of single injection (LL group), group of LPS 50mg/kg high dose of single injection (HL group), group of physiological saline injection (C group). The blood platelet count(PLT) and MPV were detected before injection as base lines,and then observe variation of PLT ,TPO ,MPV and mortality. Results   The mouse PLT was significantly lower in LL-c group among seven days than basic line (P<0.01),which was continued in the base by about 30%. The PLT were low and then high in HL and LL group. The MPV was continued significantly higher in LL-c group than basic line (P<0.01),which was augmentation and then diminution in HL group and LL group.The TPO level of LL-c group in the seventh day was higher than C group,that has significance(P<0.05).The mortality of LL-c group ,LL group and HL group were 25%(5只)、0%(0只)、70%(14只). Conclusion   The model of continuing endotoxemia TCP can be induced by consecutive low dose LPS intraperitoneal injection,the mechanism and therapy of which was futher to study.

Key words: lipopolysaccharides, endotoxemia, thrombocytopenia, thrombopoietin, mice, inbred C57BL, mean platelet volume